# MOLECULAR AND GENETIC ANALYSIS OF ALBUMIN EXTINCTION

> **NIH NIH K11** · FRED HUTCHINSON CANCER RESEARCH CENTER · 1994 · $88,166

## Abstract

We are using somatic cell and molecular genetic techniques to study loci
that regulate developmental and tissue-specific patterns of gene
expression.  Somatic hybrids formed by fusing cells of different types
generally fail to express the tissue specific products of either parent, a
phenomenon termed extinction.  Two discrete genetic loci, Tse-1 and Tse-2,
have been shown to contribute to the extinction of distinct sets of liver-
specific genes in hepatoma-fibroblast hybrids.  This is a proposal to
investigate one of these loci, Tse-2, in detail.

The aim of this project is to determine the means by which Tse-2 mediates
extinction of a group of liver genes, including albumin and alcohol
dehydrogenase.  Tse-2 has been mapped to murine chromosome 1, although
stable monochromosomal hybrids are not available to facilitate study of
Tse-2.  The initial phase of this project will use microcell-mediated
chromosome transfer to generate stable monochromosomal hybrids containing
an active Tse-2 locus.  After karyotypic and Southern blot analysis of
extinguished clones, they will be used to study the Tse-2+ phenotype in
detail, and to investigate the relationship between Tse-2 and other known
regulators of transcription of the affected genes.  These hybrids will
also be used as starting materials for cDNA subtractive cloning of the
Tse-2 transcript.

## Key facts

- **NIH application ID:** 2194400
- **Project number:** 5K11HD000936-05
- **Recipient organization:** FRED HUTCHINSON CANCER RESEARCH CENTER
- **SAM.gov UEI:** HMSNCM57QNR5
- **Principal Investigator:** AMELIA A LANGSTON
- **Activity code:** K11 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $88,166
- **Award type:** 5
- **Project period:** 1990-08-01 → 1995-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194400

## Citation

> US National Institutes of Health, RePORTER application 2194400, MOLECULAR AND GENETIC ANALYSIS OF ALBUMIN EXTINCTION (5K11HD000936-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194400. Licensed CC0.

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