# HELICASE GENE PL10 IN MOUSE GERM CELL DEVELOPMENT

> **NIH NIH K11** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1994 · $93,082

## Abstract

The murine gene PL10 was isolated in a search for genes that may play a
role in the regulation of spermatogenesis.  It is a member of the newly
defined helicase gene family.  One function proposed for members of the
helicase gene family is the regulation of gene expression through
translational control.  Phase I of this proposal will include research to
further characterize the PL10 gene, its expression and protein products, as
well as specific courses.  Phase II will be dedicated to identifying the
function of PL10.  Initially, a testis cDNA library will be screened for
full length cDNAs corresponding to the 3.2 and 3.7 kb testis-specific PL10
transcripts.  The differences between these two transcripts will be
explored through restriction mapping and sequencing.  The full length cDNAs
will then be used to synthesize the PL10 protein(s) in vitro and generate
antibodies against PL10.  The antibodies will be used to determine
temporal, tissue, cellular specificity and intracellular location of the
PL10 protein product.  If the 3.2 and 3.7 kb transcripts code for different
proteins, specific antibodies will be generated to these proteins to
further explore the differences in the two gene products.  A mouse genomic
library will be screened for the PL10 gene.  PL10's gene organization will
be characterized via restriction mapping, S1 analysis and sequencing
intron-exon boundaries.  In Phase II, two different strategies will be
employed to identify the function of the PL10 gene.  One method will be to
experimentally manipulate the expression of the PL10 gene in transgenic
animals.  The second method will involve complementation analysis of the
yeast homolog of PL10.  This research experience will provide the necessary
knowledge and skills to become an independent researcher and apply this
experience to the fields of developmental genetics and reproductive
endocrinology.

## Key facts

- **NIH application ID:** 2194448
- **Project number:** 5K11HD000974-03
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** DONNA R SESSION
- **Activity code:** K11 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $93,082
- **Award type:** 5
- **Project period:** 1992-07-01 → 1997-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194448

## Citation

> US National Institutes of Health, RePORTER application 2194448, HELICASE GENE PL10 IN MOUSE GERM CELL DEVELOPMENT (5K11HD000974-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194448. Licensed CC0.

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