# GENETIC RESISTANCE TO DISEASE THROUGH GENE TRANSFER

> **NIH NIH K11** · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · 1994 · $87,040

## Abstract

Cache Valley Virus (CVV) has been shown to be an important cause of severe
birth defects in sheep in the U.S. The fact that the virus replicates in a
number of mammals, including humans, suggests it could be a potential
teratogen in other species.  Transginec sheep which express an antisense
CVV gene will be produced to study the effects of expression of antisense
viral genes on disease resistance and prevention of congental defects.  A
pHG-CVV recombinant retrovirus will be used to transfect sheep zygotes.
PCR will be performed on transfected embryos to detect gene incorporation.
Embryos integrating the gene will be cloned by nuclear transfer to produce
multiple transgenic embryos.  Embryos will be transferred to recipient ewes
and challenged with an in utero inoculation of CVV to determine if viral
resistance has occurred, and observed for the absence of congenital
defects.

## Key facts

- **NIH application ID:** 2194480
- **Project number:** 5K11HD000987-03
- **Recipient organization:** TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
- **SAM.gov UEI:** HFT7XTHB6563
- **Principal Investigator:** JAMES N RUGILA
- **Activity code:** K11 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $87,040
- **Award type:** 5
- **Project period:** 1992-08-01 → 1996-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194480

## Citation

> US National Institutes of Health, RePORTER application 2194480, GENETIC RESISTANCE TO DISEASE THROUGH GENE TRANSFER (5K11HD000987-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194480. Licensed CC0.

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