# MYOBLAST IMPLANTATION IN SKELETAL AND CARDIAC MUSCLE

> **NIH NIH K11** · MASSACHUSETTS GENERAL HOSPITAL · 1994 · $78,046

## Abstract

This study will examine the hypothesis that the function of diseased
muscle in pediatric dystrophies may be improved by implantation of
dissociated skeletal myoblasts.  The proposal is predicated on the
observation that myoblast implantation is feasible in skeletal muscle,
allowing introduction of foreign genes and, in some models, improvement
in function.  Preliminary data in these investigations document the
feasibility of implanting genetically labelled L6 myoblasts into skeletal
muscle using both intra-arterial and intramuscular injections.  Moreover,
pilot preliminary data suggests the same cells may be implanted into
ischemic rat heart muscle.  This project intends to extend these
observations with three specific aims:  (1) study the feasibility of
using intravascular delivery of cells rather than direct intramuscular
injection, using both myocardial and skeletal muscle as host tissues; (2)
determine whether a novel method of immunomasking of donor cells may be
used to avoid rejection of donor myoblasts; and (3) ascertain whether
these myoblasts may be implanted into and function within sites of
myocardial injury where they fuse to form myotubes.  The long-term goal
is to further understand myoblast implantation, its immunobiology and the
effects of milieu upon myoblast protein expression.  Finally, this study
will evaluate the feasibility of such intravascular cellular myoblast
implantation as a therapy to augment skeletal and myocardial function in
human muscle and heart diseases.  The first phase of the PSA award will
be devoted to obtaining (a) a better understanding of the scientific
problems inherent in each of the specific aims and learning the
laboratory techniques appropriate to each section.  The second phase will
be devoted to intense research and completion of the aforementioned aims.

## Key facts

- **NIH application ID:** 2194511
- **Project number:** 5K11HD001002-02
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **SAM.gov UEI:** FLJ7DQKLL226
- **Principal Investigator:** ANN M NEUMEYER
- **Activity code:** K11 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $78,046
- **Award type:** 5
- **Project period:** 1993-02-01 → 1998-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194511

## Citation

> US National Institutes of Health, RePORTER application 2194511, MYOBLAST IMPLANTATION IN SKELETAL AND CARDIAC MUSCLE (5K11HD001002-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194511. Licensed CC0.

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