# ROLE OF YEAST MICROTUBULE PROTEIN IN CELL DIVISION

> **NIH NIH K08** · BOSTON CHILDREN'S HOSPITAL · 1994 · $77,220

## Abstract

The regulated assembly and disassembly of microtubule structures is a
major aspect of the eucaryotic cell cycle.  This dynamic behavior is
thought to be regulated in vivo by the action of microtubule-associated
proteins (MAPs).  Although MAPs have been studied extensively in vitro
little is known about their in vivo functions.  To be able to understand
the role of MAPs in vivo I am studying BIK1, the only characterized
non-motor MAP from the yeast, S cerevisiae.  The BIK1 protein associates
with microtubules in vivo, and this association appears to be restricted
to a specific interval in the cell cycle (G2/M). bikl null mutants have
phenotypes suggesting that BIK1 plays a role in several aspects of
mitotic microtubule function.

I am taking two approaches to studying BIK1 function in vivo.   The first
approach is an analysis of the regions of BIK1 required for association
with microtubules and the regions required for the cell cycle dependent
change in localization.  This will be done by examining the localization
of in vitro constructed BIK1 mutants.  I will also look for cell cycle
dependent modifications of BIK1.  The second approach is the analysis of
mutants that I have isolated (slb mutants) that require the expression
of BIKI for viability, and therefore encode overlapping functions with
BIK1.  Two of these genes will be cloned, SLB1 and SLB2.  I describe
experiments to understand the function of BIK1 through an analysis of the
physiologic defect of the bik1 slb double mutants.

The final goal of this project is to identify BIK1-like genes from human
cells and from pathogenic fungi.  This will be done by hybridization, or
by functional complementation of bik1 slb double mutants.  The isolation
of such genes would have implications for the development of
antineoplastic and antifungal drugs.

## Key facts

- **NIH application ID:** 2194527
- **Project number:** 5K08HD001009-02
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **SAM.gov UEI:** Z1L9F1MM1RY3
- **Principal Investigator:** DAVID S PELLMAN
- **Activity code:** K08 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $77,220
- **Award type:** 5
- **Project period:** 1993-07-01 → 1996-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194527

## Citation

> US National Institutes of Health, RePORTER application 2194527, ROLE OF YEAST MICROTUBULE PROTEIN IN CELL DIVISION (5K08HD001009-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194527. Licensed CC0.

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