# DEVELOPMENTAL REGULATION OF L-SELECTIN

> **NIH NIH K08** · BAYLOR COLLEGE OF MEDICINE · 1994 · $77,638

## Abstract

L-selectin is a cell surface adhesion molecule whose functions include
mediation of leukocyte-endothelial interactions during inflammation and
the "homing" of lyphocytes to peripheral lymph nodes.  The involvement of
L-selectin in lymphocyte trafficking suggests its role in the migration of
stem cells during fetal hematopoiesis or with certain leukemias.  While
the role of L-selectin in mature neutrophil function is well-established,
its role in hematopoiesis has not been extensively studied.  It is also
likely that L-selectin is developmentally-regulated, as impairment of L-
selectin-mediated function has been observed in cord blood neutrophils.
We hypothesize that: 1) L- selectin is regulated during fetal and neonatal
development and 2) L-selectin plays an influential role during normal and
dysregulated hematopoiesis.

The long term objectives of this proposal are:

1) To define the developmental regulation of functional L-selectin; and,
2) To investigate the role played by L-selectin during normal and
dysregulated hematopoiesis.  The specific aims outlined in this proposal
are structured in the following way: first, to investigate L-selectin in
the mature neutrophil; secondly, to evaluate L-selectin in the early cells
which give rise to mature neutrophils, the progenitor cells; and thirdly,
to assess the role played by L-selectin in the interaction of those
progenitors with the stromal environment during hematopoiesis.  Mature
neutrophils and neutrophil progenitors from human subjects, which include
fetuses and newborns, healthy adults, and patients with myeloid leukemia,
will be investigated using flow cytometric analysis and functional
adhesion assays.  In this way the influence of normal and dysregulated
development of L-selectin function and expression can be investigated.
Animal studies involving postmortem murine tissue as well as tissue
immortalized in cell lines at various developmental stages will be
analyzed to determine both the composition of the stroma and the influence
of developmental age on antigenic expression.  Analytical methods in these
studies will include measurement of protein expression using flow
cytometry and enzyme-linked immunosorbent assays, molecular biology
techniques to evaluate mRNA expression, and functional adhesion assays.

## Key facts

- **NIH application ID:** 2194623
- **Project number:** 1K08HD001062-01
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** JOYCE MARIE KOENIG
- **Activity code:** K08 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $77,638
- **Award type:** 1
- **Project period:** 1994-05-01 → 1999-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194623

## Citation

> US National Institutes of Health, RePORTER application 2194623, DEVELOPMENTAL REGULATION OF L-SELECTIN (1K08HD001062-01). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194623. Licensed CC0.

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