# REGULATION OF LIMB CHONDROGENESIS

> **NIH NIH R01** · UNIVERSITY OF IOWA · 1993 · $267,791

## Abstract

The developing limb is a useful model for studies on the mechanisms
involved in tissue patterning during development.  This proposal
is concerned with the processes that lead to the differentiation
of cartilage elements in their proper spacial relationships in the
developing avian and mouse limb.  By means of grafting studies in
ovo and tissue culture studies, the dynamics and inductive
properties of the limb ectoderm will be examined.  An ectodermally
derived factor that inhibits cartilage differentiation will be
characterized.  By use of a high density culture system in which
chondrogenesis occurs, the mechanism by which specific interactions
between mesenchymal cells promote chondrogenesis will be analyzed.
Particular attention will be placed on extracellular matrix
receptors and changes in the organization of the actin cytoskeleton
in the initiation of chondrogenesis.  Studies will also be
concerned with the nature of a precartilage extracellular matrix
molecule and its role in chondrogenesis.  The role of insulin-like
growth factor I in chondrogenesis and in mediating defects in
chondrogenesis in the droopy ear mouse mutant model will be studied
in cell cultures by use of blocking antibody to IGF-I and molecular
probes.  Finally, the mechanism by which cell shape changes
modulate the expression of cartilage specific genes will be
examined.  Particular attention will be placed on translation level
regulation of the synthesis of various collagen types.

## Key facts

- **NIH application ID:** 2194896
- **Project number:** 5R01HD005505-21
- **Recipient organization:** UNIVERSITY OF IOWA
- **SAM.gov UEI:** Z1H9VJS8NG16
- **Principal Investigator:** KARLA J DANIELS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $267,791
- **Award type:** 5
- **Project period:** 1978-03-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2194896

## Citation

> US National Institutes of Health, RePORTER application 2194896, REGULATION OF LIMB CHONDROGENESIS (5R01HD005505-21). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2194896. Licensed CC0.

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