# IDENTIFICATION OF GENES INTERACTING WITH C ELEGANS

> **NIH NIH F32** · UNIVERSITY OF WISCONSIN-MADISON · 1993 · $22,700

## Abstract

The aim of the proposed research is a better understanding of how
inductive events function in the development of a multicellular
organism. This work will focus on genetic and molecular analysis of
factors that interact with the product of the C.elegans gen glp-1 (for
germ line proliferation). This gene encodes a transmembrane protein that
functions in the development of two major tissues in C.elegans; the germ
line and the anterior pharynx. I propose to use two molecular approaches
to identify genes that specifically interact with the conserved
intracellular portion of glp-1. This domain of the protein contains the
conserved repeats homologous to the cell cycle an tissue differentiation
genes in many organisms. The first method will employ a yeast genetic
system that allows selection for physically interacting proteins. The
other method relies on construction of labeled fusion proteins to
directly probe C.elegans expression libraries. C.elegans is an excellent
system for this type of study because once clones are identified they
can be analyzed using the genetics of the organism. In a genetic screen
I will use one of the many well characterized mutant alleles of glp-1 to
look for enhancers of weak glp-1 phenotypes. In this way I hope to
obtain a better understanding of not only C.elegans development but of
the role of cell-cell interactions in general.

## Key facts

- **NIH application ID:** 2195920
- **Project number:** 5F32HD007604-02
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **SAM.gov UEI:** LCLSJAGTNZQ7
- **Principal Investigator:** SAMUEL T HENDERSON
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $22,700
- **Award type:** 5
- **Project period:** 1993-09-01 → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2195920

## Citation

> US National Institutes of Health, RePORTER application 2195920, IDENTIFICATION OF GENES INTERACTING WITH C ELEGANS (5F32HD007604-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2195920. Licensed CC0.

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