# ESTROGEN REGULATION OF GROWTH AND FUNCTION

> **NIH NIH R37** · UNIVERSITY OF WISCONSIN-MADISON · 1994 · $206,196

## Abstract

Extrogenic hormones coordinate a variety of physiological responses that
are essential for reproduction.  In mammals, including humans, the uterus
and the pituitary gland contain estrogen responsive cells that are the
subject of the proposed studies.  On the basis of recent investigations
estrogen receptors appear to be nuclear proteins probably bound to other
nuclear constituents with or without the estrogenic ligand.  This proposal
details studies using aqueous two-phase partitioning to examine the changes
in structure of the receptor upon binding estrogenic compounds, and to
provide some insight into the thermodynamics of such changes.  Development
of minichromosome systems using viral vectors will provide a means to
isolate and amplify specific gene sequences and their accompanying
chromatin proteins.  This is particularly important because current data
suggests that nucleotide sequence of DNA or nuclear proteins alone do not
provide the specificity required to explain the complex action of
estrogens.  Receptor concentration is also essential to the physiological
response to estrogen.  Studies will be carried out to assess the biological
regulation of development of estrogen receptors in the uterus and
pituitary.  One of the manifestations of estrogen responsiveness is the
growth of responsive tissues.  Two approaches will be used to find genes
involved in growth that are regulated by estrogen.  the protooncogenes are
genes that appear to be related intimately to growth and development.
Cloned probes for these genes are available and can be used to determine if
and how these genes are related to estrogen regulated growth.  A second
approach will be to use cDNA libraries made from estrogen stimulated and
control tissues of rats that differ in their growth response to estrogens.
This also may provide a means for identifying growth essential genes that
respond to estrogenic hormones.  If either of these methods identifies an
estrogen responsive gene, it will be used for studies on chromatin
interaction with estrogen receptor.

## Key facts

- **NIH application ID:** 2196570
- **Project number:** 5R37HD008192-22
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **SAM.gov UEI:** LCLSJAGTNZQ7
- **Principal Investigator:** JACK GORSKI
- **Activity code:** R37 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $206,196
- **Award type:** 5
- **Project period:** 1978-05-01 → 1996-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2196570

## Citation

> US National Institutes of Health, RePORTER application 2196570, ESTROGEN REGULATION OF GROWTH AND FUNCTION (5R37HD008192-22). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2196570. Licensed CC0.

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