# MECHANISMS OF THYROID HORMONE ACTION IN DEVELOPMENT

> **NIH NIH R01** · DARTMOUTH COLLEGE · 1994 · $188,635

## Abstract

DESCRIPTION (Adapted from the applicant's abstract):  The long term goal of
this research project is to elucidate the cellular processes involved in
thyroid hormone (TH) action.  Specifically, this proposal addresses the
molecular mechanisms which regulate TH metabolism in developing vertebrate
species.  In the fetus, as in the adult, specific iodothyronine deiodinases
convert thyroxine and other thyroid hormones into active and inactive
metabolites.  Recently, a cDNA which encodes the type I rat liver 5'-
deiodinase (5'D) was isolated and its sequence determined.  Thus, studies
at a molecular level aimed at investigating the mechanisms by which
deiodinases are regulated during development can now be undertaken. Two
animal models will be employed:  the rat, which is traditionally used as an
experimental model system for studying mammalian TH economy, and the frog,
which offers the major advantage that its larval form is free swimming and
thus readily amenable to experimental manipulation.  Specific Aim 1 is to
isolate and characterize cDNAs for the 5'D and 5D present in tadpoles.  In
Specific Aim 2 and #4 these tadpole and rat cDNAs will be used as molecular
probes to characterize the pretranslational regulation of the deiodinase
enzymes at various stages of development by quantitating mRNA levels and by
in situ hybridization.  The role of TH and other factors in regulating 5'D
and 5D mRNA levels will be investigated in rat fetuses and tadpoles
(Specific Aim 3) and in cultured fetal rat hepatocytes and tadpole tail
tips (Specific Aim 5).  The effect of TH on 5'D and 5D gene transcription
will then be determined using a transcription assay (Specific Aim 6).
Specific Aim #7 is to isolate from frog and rat genomic libraries the genes
which encode the 5'D and 5D and their associated DNA flanking regions.

## Key facts

- **NIH application ID:** 2196645
- **Project number:** 5R01HD009020-19
- **Recipient organization:** DARTMOUTH COLLEGE
- **SAM.gov UEI:** EB8ASJBCFER9
- **Principal Investigator:** VALERIE Anne GALTON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $188,635
- **Award type:** 5
- **Project period:** 1978-05-01 → 1996-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2196645

## Citation

> US National Institutes of Health, RePORTER application 2196645, MECHANISMS OF THYROID HORMONE ACTION IN DEVELOPMENT (5R01HD009020-19). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2196645. Licensed CC0.

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