# MECHANISMS OF IMPLANTATION OF THE BLASTOCYST

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA AT DAVIS · 1994 · $217,845

## Abstract

Cytological, immunohistological and immunocytological methods will be
used to investigate the role of different trophoblast populations during
implantation in the macaque, and to compare these observations with those
in two species, the rat and horse, exhibiting different patterns of
epithelial and stromal invasion.  The role of fetal-maternal cell fusion
in expansion of trophoblast along the plane of the epithelium will be
studied in the macaque using cytological and cell marker techniques.  The
extent of linkage between cytotrophoblast cells and cytotrophoblast cells
and syncytial trophoblast will be studied using antibodies to junctional
complex constituent proteins in this species.  DNA synthesis in
trophoblast populations will be examined using 3H thymidine incorporation
in vitro in macaque implantation sites.  The role of selected cell
adhesion molecules in interaction of trophoblast and uterine tissues will
be followed using antibody methods during implantation and early
placentation in the macaque and rat.  As a means of characterizing
different trophoblast populations and providing functional implications
the distribution of lysosomal and nonlysosomal lytic enzymes will be
mapped in macaque trophoblastic plate to anchoring villous stages.
Surface modifications and differentiation of equine girdle cells will be
examined as they relate to migration of these cells into the endometrium.
Adhesion molecules also will be studied in relationship to segregating
cell populations in the uterus and trophoblast of the rat during
implantation and early placentation.  Adhesion molecules between
trophoblast cells and endothelial cells will be localized to establish
their role in formation of vascular patterns in the macaque and rat, and
the manner in which equine trophoblast cells invade lymphatic vessels but
not capillaries will be examined.  Finally, embryos obtained during the
macaque studies will be used to examine the manner of formation of the
secondary yolk sac and the contribution of the prochordal plate and
notochordal process to the establishment of definitive intraembryonic
endoderm.  It is believed that these studies can make a substantial
contribution to our understanding of the way in which trophoblast cells
can interact with the endometrium during the crucial implantation stages
preceding placentation.

## Key facts

- **NIH application ID:** 2196715
- **Project number:** 5R01HD010342-19
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **SAM.gov UEI:** TX2DAGQPENZ5
- **Principal Investigator:** ALLEN C ENDERS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $217,845
- **Award type:** 5
- **Project period:** 1977-04-01 → 1997-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2196715

## Citation

> US National Institutes of Health, RePORTER application 2196715, MECHANISMS OF IMPLANTATION OF THE BLASTOCYST (5R01HD010342-19). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2196715. Licensed CC0.

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