# GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO

> **NIH NIH R01** · DUKE UNIVERSITY · 1994 · $156,319

## Abstract

During the previous grant period our laboratory identified several
Transforming Growth Factor-Beta (TGF-beta) sub-types in the ovary and
demonstrated significant interrelationships between TGF-B and Follicle-
Stimulating Hormone (FSH) in regulating ovarian cellular function.  We
also detected growth inhibitory activity in the conditioned culture
medium of porcine granulosa cells (GC) which was not TGF-beta and by
molecular weight estimate does not represent any of the known growth
factors or their binding proteins.  Specifically, current results
indicate the presence of a putative small-molecular-weight polypeptide
inhibitory to 3H-thymidine uptake/cell proliferation and activity which
induces apoptosis (programmed cell death) in these cells.  Since our
knowledge of the locally acting biochemical factors regulating the
cessation of ovarian follicular development is virtually non-existent,
the isolation and identification of new/novel factors which inhibit
follicle cell growth and/or induce programmed cell death would be very
significant research advance in this area.  Accordingly, the specific
aims of this proposal are to elucidate at the molecular level the
characteristics of the causative effector(s): 1) to determine whether the
same factor or different factors are responsible for the growth
inhibitory and apoptotic activities; 2) to isolate the putative growth
inhibitory polypeptide and clone the gene for it - a) isolate by
preparative and analytical HPLC, b) determine amino acid sequence, c)
generate antibodies, d) screen cDNA libraries with antibodies or labelled
oligonucleotides, and e) determine nucleotide sequence; 3) to analyze
genomic DNA and mRNA with respect to species and cell distribution - a)
prepare labelled cDNA probes, b) Southern blot analysis of genomic DNA,
c) Northern blot analysis of mRNA, including that from human ovarian
cells, to determine transcript(s) size and patterns of cellular
expression.

The long term objective of these studies is to provide a basic
understanding of ovarian cellular function which will eventually be
applicable to the problem of fertility regulation.

## Key facts

- **NIH application ID:** 2196843
- **Project number:** 2R01HD011827-13A3
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** DAVID W SCHOMBERG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $156,319
- **Award type:** 2
- **Project period:** 1978-04-01 → 1996-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2196843

## Citation

> US National Institutes of Health, RePORTER application 2196843, GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO (2R01HD011827-13A3). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2196843. Licensed CC0.

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