# PEPTIDE HORMONES OF REPRODUCTION:  CHEMISTRY AND ASSAY

> **NIH NIH R01** · MASSACHUSETTS GENERAL HOSPITAL · 1994 · $244,594

## Abstract

The objectives of this project are to establish the structural
requirements for biological activity in the human glycoprotein hormones,
luteinizing hormone (hLH) and human chorionic gonadotropin (hCG).  Under
Aim 1, using previous NMR structure of LMB (38-57) as a baseline, Dr.
Keutmann will continue NMR analysis to define structure.  They then will
use a monoclonal antibody as a "surrogate receptor" in analysis of this
ordered structure, in association with a binding site, using the
technique of transferred nuclear-Overhauser effect spectroscopy.  Under
Aim 2, to define more fully the surface topography.  Under Aim 2, to
define more fully the surface topography of the receptor-associated
peptide, they will determine the crystal structure of the loop in
association with a Fab fragment of monoclonal antibody specific to the
loop.  Under Aim 3, they will use site-directed mutagenesis to verify
that biologically- significant modifications in the peptide are also
significant in the whole hormone, and might also exert effects on
structure elsewhere in the overlapping adjacent regions.

## Key facts

- **NIH application ID:** 2196939
- **Project number:** 2R01HD012851-14A1
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **SAM.gov UEI:** FLJ7DQKLL226
- **Principal Investigator:** HENRY T KEUTMANN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $244,594
- **Award type:** 2
- **Project period:** 1979-09-30 → 1997-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2196939

## Citation

> US National Institutes of Health, RePORTER application 2196939, PEPTIDE HORMONES OF REPRODUCTION:  CHEMISTRY AND ASSAY (2R01HD012851-14A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2196939. Licensed CC0.

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