# GONADOTROPIN ACTION

> **NIH NIH R01** · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · 1994 · $165,488

## Abstract

The long-term objective of this project is to identify how the
interaction between the gonadotropins and their receptors results in
signal transduction.  Particular emphasis will be placed on developing
a model of receptor structure and identifying changes in receptor
structure which occur after binding of hormone agonists and antagonists.
Specific aims are:  1) to identify the disulfide bonds of the luteinizing
hormone receptor (LHR) and follicle stimulating hormone receptors (FSHR),
2) to characterize changes in the extracellular N-terminus and
extracellular portions of the transmembrane domain which occur following
binding of human chorionic gonadotropin (hCG) and hFSH, 3) to
characterize changes in the intracellular portions of the receptors
caused by hormone binding, and 4) to distinguish changes in the receptors
which are essential for hormone activity from those which occur on
binding of antagonists.  Specific aim #1 depends on the use of
mutagenesis to introduce methionine residues into various portions of the
LHR and FSHR.  This will permit the receptors to be cleaved with CNBr
into known fragments.  The locations of the disulfide bonds will be
determined from the molecular weights of the fragments as determined in
Western blots.  Specific aim #2 depends on incorporation f radioiodine
into specific tyrosine residues which are on the extracellular surface
of the receptor.  These will be identified by immunoprecipitation of the
receptors, cleaving the receptors with CNBr, and monitoring radioactivity
in specific peptide fragments.  Studies are planned to determine if the
presence of hormone alters the incorporation of radioiodine into these
tyrosines.  Procedures for specific aim #3 are similar to those of aim
#2 except that the cytoplasmic surface of the receptor will be studied.
In specific aim #4 the influences of deglycosylated hormones and other
antagonists on tyrosine radiolabeling will be compared with those of the
native hormones.  These studies should improve knowledge of the manner
in which the glycoprotein hormones initiate their biological responses.
Further, since the gonadotropin receptors are part of a larger class of
similar proteins which participate in signal transduction, information
learned should be widely applicable.

## Key facts

- **NIH application ID:** 2197140
- **Project number:** 5R01HD014907-11
- **Recipient organization:** UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
- **Principal Investigator:** WILLIAM R MOYLE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $165,488
- **Award type:** 5
- **Project period:** 1983-08-01 → 1996-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197140

## Citation

> US National Institutes of Health, RePORTER application 2197140, GONADOTROPIN ACTION (5R01HD014907-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197140. Licensed CC0.

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