# STUDIES OF GONADOTROPINS

> **NIH NIH P01** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 1994 · $1,030,843

## Abstract

This Program Project entitled, "Studies of Conadotropins" is composed of
four research projects and two core facilities. Its central theme is to
improve the understanding of gonadotropin structure-function relationships
and to improve methods to quantify gonadotropins in studies of human
reproduction. The first research project entitled, "Hormone Structure and
Function" will be directed by Dr. William Moyle and applies the techniques
of genetic engineering to make modifications in gonadotropin gene
structure, so the expressed products can be evaluated for alterations of
function. Alterations to be studied include hormone receptor binding and
biological activity, and it is expected that the results will define the
amino acids that contribute to the structure of antibody binding sites.
The second research project entitled, "Protein Chemistry of Gonadotropins
and Fragments" will be directed by Dr. Steven Birken and applies the
techniques of protein chemistry to the genetically-altered proteins
produced by Project I and also attempts to solve the location of the
disulfide bridges in the native structure of Gonadotropin subunits, as well
as the origins and structure of gonadotropin fragments that are employed as
disease markers. Project III entitled, "Biophysical and Immunochemical
Studies of Gonadotropins" will be directed by Dr. Canfield, and this will
serve as the interface of all disciplines in the program with a special
emphasis on protein physical chemistry and immunology. The goal is to
increase understanding of the three-dimensional structure of gonadotropins
by three-dimensional model building, NMR studies, protein crystallography
experiments, and by producing novel antibodies to gonadotropins, whose
defined epitopes provide useful information about the surface arrangement
of amino acids. Project IV entitled, "Clinical Applications of Gonadotropin
Measurement" will be directed by Dr. John O'Connor, and its goal is to
apply immunologic techniques to improve clinical research measurements
related to early pregnancy loss, ovulation, and other reproductive events.
This program will also include an Administrative Core Facility, directed by
Dr. Canfield, and a Tissue Culture and Hybridoma Core, directed by Dr.
Alexander Krichevsky. Both will provide services to all four research
projects.

## Key facts

- **NIH application ID:** 2197204
- **Project number:** 5P01HD015454-14
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **SAM.gov UEI:** QHF5ZZ114M72
- **Principal Investigator:** ROBERT E CANFIELD
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $1,030,843
- **Award type:** 5
- **Project period:** 1981-07-01 → 1995-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197204

## Citation

> US National Institutes of Health, RePORTER application 2197204, STUDIES OF GONADOTROPINS (5P01HD015454-14). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197204. Licensed CC0.

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