# OOGENESIS AND EARLY EMBRYOGENESIS IN DROSOPHILA

> **NIH NIH R37** · PRINCETON UNIVERSITY · 1994 · $219,137

## Abstract

The proposed experiments focus on interactions between maternal and
zygotic components during cellularization and gastrulation in Drosophila
development.  We will study two genes [folded gastrulation (=fog) and
concertina (=cta)] which are required for normal formation of the
posterior midgut, characterizing the morphological phenotypes of both
loci in terms of cell shape and morphogenetic movements.  By injecting
wild type cytoplasm into eggs from cta femase, we will test whether cta=
activity is localized to the same regions of the embryo known to
required fog expression.  Both cat and fog will be cloned.  In situ
hybridization and antibodies to their respective protein products will
be used to determine whether the gene products are localized only to the
posterior regions of the embryo affected by each mutant.  The probes
will also be used to define in molecular terms the epistatic
relationships between the two loci.

We will also study two zygotically active loci (nullo 6F1-2 and halo,
22A) which cause abnormalities in actin and lipid distribution early
during cellularization.  The phenotype of mutants and wild type embryos
processes will be characterized morphologically and genetic mosaics will
be used to determine the autonomy of the phenotypes and the specificity
of the gene products for embryogenesis.  Interactions between both loci
and maternal effect mutations shown to affect cellularization will be
analyzed.  The nullo locus will be cloned and the distribution of its
protein product during the establishment of the hexagonal actin arrays
will be described.

## Key facts

- **NIH application ID:** 2197228
- **Project number:** 4R37HD015587-14
- **Recipient organization:** PRINCETON UNIVERSITY
- **SAM.gov UEI:** NJ1YPQXQG7U5
- **Principal Investigator:** ERIC F. WIESCHAUS
- **Activity code:** R37 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $219,137
- **Award type:** 4N
- **Project period:** 1981-08-01 → 1999-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197228

## Citation

> US National Institutes of Health, RePORTER application 2197228, OOGENESIS AND EARLY EMBRYOGENESIS IN DROSOPHILA (4R37HD015587-14). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197228. Licensed CC0.

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