# AUTONOMIC INTERACTIONS IN DEVELOPING HEART

> **NIH NIH R01** · AUGUSTA UNIVERSITY · 1994 · $126,131

## Abstract

The objective of this project is to determine the mechanism(s) and temporal
sequence involved in the commitment of cardiac neural crest cells to
mesenchymal, neuronal and non-neuronal cell lines which are essential for
normal development of the heart.  It is proposed that the decisions made by
the cardiac neural crest cells are analogous to those made in the
neurogenic region of invertebrate embryos where specific sets of proneural
genes are expressed to control the determination of cell lineage.  One of
the proneural genes of the achaete-scute complex (T3), will be
investigated.  This gene has been well-characterized in Drosophila and a
potential homologues have been identified in chick (CASH-1, C-Notch).  The
present experiments will characterize the avian homologue of this genes and
determine whether it has a role in establishing cell lineage in the cardiac
neural crest.  Avian cDNA clones will be obtained and characterized.  The
chick clones and monoclonal antibodies, generated to various regions of the
protein products will be used to determine when these genes are expressed
in the cardiac neural crest using Northern analysis, in situ hybridization,
and immunohistochemistry.  Expression of the genes in the cardiac neural
crest will be altered in vitro and in vivo.  The defects caused by modified
expression of the genes will be correlated with a quantitative assessment
of cardiac neural crest derivatives.  Understanding these molecular events
will provide a rationale for testing possible genetic and environmental
causes of congenital cardiovascular diseases.

## Key facts

- **NIH application ID:** 2197398
- **Project number:** 5R01HD017063-10
- **Recipient organization:** AUGUSTA UNIVERSITY
- **SAM.gov UEI:** N4WWJC8T2593
- **Principal Investigator:** Margaret Loewy Kirby
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $126,131
- **Award type:** 5
- **Project period:** 1982-04-01 → 1997-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197398

## Citation

> US National Institutes of Health, RePORTER application 2197398, AUTONOMIC INTERACTIONS IN DEVELOPING HEART (5R01HD017063-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197398. Licensed CC0.

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