# IN VITRO EXPRESSION OF HORMONE-REGULATED GENES

> **NIH NIH R01** · BAYLOR COLLEGE OF MEDICINE · 1994 · $249,578

## Abstract

Diabetes mellitus is a complex disease in which the primary clinical
symptom is abnormally high levels of plasma glucose.
It may affect up to 5% of the human population.  The major regulatory
hormones that control glucose homeostasis are insulin and glucagon. Both
hormones are expressed only in islet cells of the pancreas.

In order to understand why insulin genes are only expressed in islet cells,
it is necessary to develop a cell-free system to identify all the necessary
components required for the expression of this important gene. Once these
components are identified, one can then study their mechanism of action. In
this proposal, we design experiments to identify, isolate and study the
mechanism of action of transcription factors required for the expression of
the insulin gene. These studies will involve various aspects of
biochemistry, protein chemistry, molecular biology, some immunology and
some physiology.

It is expected that the results derived from this project will be relevant
to the better understanding of the insulin gene regulation. In addition,
the following proposal should also be pertinent in elucidating a more
precise hypothesis for the regulation of gene expression during
differentiation, development and cancer metastasis.

## Key facts

- **NIH application ID:** 2197429
- **Project number:** 5R01HD017379-11
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** Ming-Jer Tsai
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $249,578
- **Award type:** 5
- **Project period:** 1983-02-01 → 1995-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197429

## Citation

> US National Institutes of Health, RePORTER application 2197429, IN VITRO EXPRESSION OF HORMONE-REGULATED GENES (5R01HD017379-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197429. Licensed CC0.

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