# TRANSPORT OF PROTEIN IN NEONATAL ILEUM AND PEYER'S PATCH

> **NIH NIH R37** · BOSTON CHILDREN'S HOSPITAL · 1994 · $252,884

## Abstract

This project is focused on two intestinal epthelial cells that are
specialized for uptake of luminal macromolecules--the absorptive cell of
neonatalileum, and the M cell of Peyers's patch.  Although they differ
dramatically in structure and function, both cell types provide valuable
models for study of epithelial membrane traffic, intracellular sorting, and
transepithelial transport.

In neonatal ileum, tracer proteins and immunocytochemistry will be used to
detect sorting of physiologic and foreign adsorptive ligands and receptors
at luminal cell surfaces and in apical endosomal tubules, and selective
transepithelial transport.  Subcellular fractions enriched in tubules will
be analysed biochemically and used to raise organelle-specific antibodies,
that in turn will serve as membrane markers.  The independent recycling of
apical and basolateral endocytic membranes and the convergence of these
polarized pathways in the central lysosomal system will be demonstrated
using tracer ligands and lysosome-specific antibodies.

Transport of antigens across rat and rabbit M cells will be assessed in
quantitative studies using ultrastructural and fluorescent tracers and
radiolabeled probes.  Relative efficiencies of transport of soluble vs.
adherant molecules and particles, and specific types of adherent monomeric
or polymeric molecules will be systematically compared.  M cell membrane
domains and the transepithelial transport pathway will be further explored
using domain-specific antibodies and probes specific for acidic
compartments, along with tracer proteins.

New information on membrane traffic and selective and nonselective protein
transport in these cells will clarify their roles in epithelial barrier
function and mucosal immunity.

## Key facts

- **NIH application ID:** 2197486
- **Project number:** 5R37HD017557-13
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **SAM.gov UEI:** Z1L9F1MM1RY3
- **Principal Investigator:** MARIAN R. NEUTRA
- **Activity code:** R37 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $252,884
- **Award type:** 5
- **Project period:** 1983-07-01 → 1996-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197486

## Citation

> US National Institutes of Health, RePORTER application 2197486, TRANSPORT OF PROTEIN IN NEONATAL ILEUM AND PEYER'S PATCH (5R37HD017557-13). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197486. Licensed CC0.

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