# UTERUS--CYCLIC HORMONAL CONTROL MECHANISMS

> **NIH NIH P01** · UNIV OF MASSACHUSETTS MED SCH WORCESTER · 1993 · $751,498

## Abstract

Although the hormonal mechanisms of the hypothalamic-pituitary-ovarian axis
have been well studied in the primate, the growth, differentiation and
response of the endometrium and uterus in menstruating primates remain
largely undefined.  To provide an experimental model applicable to the
human uterus, an interdisciplinary study of the rhesus monkey uterus will
continue to be used to determine cyclic endometrial patterns of cell
growth, differentiation, and response.  The model that is used is an
ovariectomized rhesus monkey in which the hormonal milieu is provided by
the timed insertion and withdrawal of silastic capsules containing estrogen
or progesterone.  In this fashion we simulate a standard artificial
menstrual cycle (SAMC) or a manipulated artificial menstrual cycle (MAMC).
On the appropriate days of either cycle, tissue specimens of endometrium
will be obtained and, in some cases, tissues and blood samples will be
obtained after an infusion of radiolabeled estrogens or androgens.  The
tissues so obtained will be studied with the following approaches:  Zonal-
dependent and cell-type specific changes in estradiol and progesterone
receptors and potential autocrine/paracrine regulatory factors by
immunohistochemistry, endometrial zonal proliferation patterns as assessed
by immunohistochemistry of the Ki67 antigen, in situ hybridization and
Northern analyses of ER and PR and potential autocrine/paracrine factors;
estrogen uptake and metabolism, the role of SHBG and zonal cell-type
immunohistochemical localization of aromatase enzymes; effects of E and P
on endometrial prostaglandin production in vivo and in vitro and their
mechanism of action; and zonal-dependent and cell-type specific changes in
3H-thymidine labeling indices and morphometric and structural analyses at
the LM and EM level.  Human disorders of fertility, endometrial
hyperplasia, and endometriosis may be better understood by a thorough and
systematic analysis of the factors that regulate primate endometrial
proliferation and response.

## Key facts

- **NIH application ID:** 2197986
- **Project number:** 5P01HD020290-08
- **Recipient organization:** UNIV OF MASSACHUSETTS MED SCH WORCESTER
- **SAM.gov UEI:** MQE2JHHJW9Q8
- **Principal Investigator:** CHRISTOPHER LONGCOPE
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $751,498
- **Award type:** 5
- **Project period:** 1985-08-01 → 1994-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2197986

## Citation

> US National Institutes of Health, RePORTER application 2197986, UTERUS--CYCLIC HORMONAL CONTROL MECHANISMS (5P01HD020290-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2197986. Licensed CC0.

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