# STEROID MODULATION OF ADRENERGIC SIGNAL TRANSDUCTION

> **NIH NIH R01** · UNIVERSITY OF CHICAGO · 1994 · $163,733

## Abstract

Although temporally related to the near term fall in progesterone (Pr)
and rise in estradiol (E2) observed in the pregnant rat, the hormonal
control for the loss of beta adrenergic responsiveness and modulation of
beta-2 adrenergic receptor (B2AR) signal transduction events observed
near term in the pregnant rat are unknown at this time. The proposed
experiments will test the hypothesis that E2 and/or Pr regulate beta-2
adrenergic responsiveness by directly effecting B2AR expression and/or
B2AR signal transduction mechanisms. This hypothesis will be tested in a
systematic fashion using rat myometrial tissue obtained during normal
gestation, myometrial tissue obtained from pregnant rats undergoing
steroid hormone manipulation in vivo, and cultured rat uterine myocytes
directly stimulated in vitro with steroid hormones.
This revised, competitive renewal research grant application is to
evaluate the effects of gestation and steroid hormones on: 1) the
expression of myometrial beta-2 adrenergic receptors utilizing
radioligand binding studies and Northern blots, 2) guanine nucleotide
regulatory protein (Gs) function, Gs-alpha subunit MRNA levels, and
protein expression, 3) adenylate cyclase (AC) activity, AC MRNA levels,
and protein expression, and 4) protein kinase A (CAMP-dependent protein
kinase) activity, myocyte PKA target peptides, PKA MRNA levels, and
protein expression. The proposed studies will further characterize
adrenergic signal transduction mechanisms in pregnant myometrial tissue,
with particular emphasis on the elucidation of those events regulated by
these steroid hormones as parturition approaches.

## Key facts

- **NIH application ID:** 2198440
- **Project number:** 5R01HD022063-07
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **SAM.gov UEI:** ZUE9HKT2CLC9
- **Principal Investigator:** Mark Phillippe
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $163,733
- **Award type:** 5
- **Project period:** 1987-06-01 → 1996-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198440

## Citation

> US National Institutes of Health, RePORTER application 2198440, STEROID MODULATION OF ADRENERGIC SIGNAL TRANSDUCTION (5R01HD022063-07). Retrieved via MORVS 2026-10-26 from https://api.morvs.ai/grant/nih/2198440. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*