# HORMONAL CONTROL OF C-KINASE AND CORPUS LUTEUM FUNCTION

> **NIH NIH R01** · UNIVERSITY OF KANSAS MEDICAL CENTER · 1994 · $105,053

## Abstract

The mammalian corpus luteum is a   transient gland.  During the menstrual
or estrous cycle, it develops, secretes progesterone for a period of time
characteristic of the species, and then declines.  In the human and the
cow, the tropic factors which regulate luteal function have been
demonstrated to be predominantly luteinizing hormone (LH) and human
chorionic gonadotropin.  However, the mechanisms by which the effects of
LH are propagated at the cellular and molecular level remain a mystery.
It is well recognized that progesterone secretion is closely coupled to
the ability of LH to increase cAMP and activate cAMP-dependent protein
kinases but recent evidence implicates another pathway resulting in the
phosphorylation of ovarian proteins.  This pathway is linked to the
hydrolysis of inositol phospholipids, calcium flux and stimulation of
calcium dependent protein kinases; one such kinase, protein kinase C, has
been implicated in the regulation of a variety of cellular activities in
ovarian cells.  Importantly, recent evidence has clearly demonstrated the
existence of a family of protein kinase C enzymes in hormone-responsive
tissues, each displaying different biochemical properties,
tissue-distribution and developmental expression.  The specific aims of
the proposed research are to identify and characterize protein kinase C
isozymes in the bovine corpus luteum.  This proposal will focus on fully
characterizing ovarian protein kinase C isozymes and documenting their
tissue distribution and developmental expression under physiologically
relevant conditions in the cow.  Measurements of protein kinase activity
will be compared with levels of mRNA for specific protein kinase C
isozymes.  Cultures of bovine small and large luteal cells will provide
needed information on the hormonal regulation of protein kinase C
isozymes under defined conditions.  These studies are expected to improve
our knowledge of the mechanism of action of LH and other hormones which
control the development and function of the corpus luteum.  Understanding
these regulatory mechanisms may lead to improved methods of fertility
control.

## Key facts

- **NIH application ID:** 2198501
- **Project number:** 5R01HD022248-08
- **Recipient organization:** UNIVERSITY OF KANSAS MEDICAL CENTER
- **SAM.gov UEI:** YXJGGNC5J269
- **Principal Investigator:** JOHN S DAVIS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $105,053
- **Award type:** 5
- **Project period:** 1986-08-01 → 1996-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198501

## Citation

> US National Institutes of Health, RePORTER application 2198501, HORMONAL CONTROL OF C-KINASE AND CORPUS LUTEUM FUNCTION (5R01HD022248-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198501. Licensed CC0.

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