# INTESTINAL LIPOPROTEINS IN THE NEONATE

> **NIH NIH R01** · ARKANSAS CHILDREN'S HOSP (LITTLE ROCK) · 1994 · $167,889

## Abstract

Apolipoprotein are surface components of lipoprotein particles and serve
essential functions in the secretion, metabolism, and receptor-mediated
uptake of thee particles.  Delineation of the factors that regulate
apolipoprotein gene expression in the small intestine of the developing
mammal has been difficult because of the simultaneous interaction of
several potential regulatory factors and the lack of a suitable animal
model.  The elucidation of these regulatory mechanisms is essential
because of the importance of these proteins in lipoprotein biogenesis and
metabolism in the neonate.  An in vitro neonatal swine model has been
developed which offers the advantages of ease of manipulation and
significant homology to the human with regard to lipoprotein metabolism
and timing of gastrointestinal maturation.  Techniques have been
developed to allow study of apolipoprotein expression at the pre-, co-,
and post-transnational levels.  Studies to date have identified distinct
patterns of apo B, A-I, and A-IV expression by dietary and biliary lipid
absorption in the intact animal in vivo and by epidermal growth factor,
insulin, and hydrocortisone in developmental expression defined.
Intestinal expression of apo C-II mRNA is limited to the neonatal jejunum
and appears to be highly regulated by lipid absorption.   A novel cell
line, IPEC-1, derived from the small intestine of an unsuckled newborn
piglet, has been characterized with regard to apolipoprotein basolateral
membrane secretion of lipoproteins when grown on collagen-coated filters.
These cells also exhibit increased secretion of apo B, A-I, and A-IV in
response to incubation with fatty acid.  Furthermore, these cells absorb
free fatty acid, esterify it into intracellular triglyceride, cholesteryl
ester, and phospholipid, and secrete lipoproteins containing these
lipids.  The overall aim of this proposal is to further define the
effects of dietary lipid and the hormonal milieu on apolipoprotein gene
expression in the small intestine of the neonatal mammal.  An integrated
approach using intact animals and the IPEC-1 intestinal cell line will
achieve the following: 1) Determine whether changes in enterocyte
apolipoprotein hormone treatment are accompanied by coordinate changes
in lipoprotein composition and secretion in cultured IPEC-1 cells.  2)
Determine the effects of feeding short- (24 hours) and long-term (1 week)
diets of varying fat composition (saturated versus polyunsaturated,
medium versus long chain length) with and without cholesterol on
intestinal and hepatic apolipoprotein gene expression and plasma
apolipoprotein and lipoprotein profiles using the in vivo newborn pig
model.  This information should ultimately guide the optimal provision
of dietary lipid to the neonate with particular relevance to the lipid
composition of infant formulas.

## Key facts

- **NIH application ID:** 2198568
- **Project number:** 2R01HD022551-06A2
- **Recipient organization:** ARKANSAS CHILDREN'S HOSP (LITTLE ROCK)
- **Principal Investigator:** DENNIS Darrel BLACK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $167,889
- **Award type:** 2
- **Project period:** 1987-02-01 → 1997-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198568

## Citation

> US National Institutes of Health, RePORTER application 2198568, INTESTINAL LIPOPROTEINS IN THE NEONATE (2R01HD022551-06A2). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198568. Licensed CC0.

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