# MECHANISMS OF CYCLIC LH AND FSH RELEASE

> **NIH NIH R01** · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · 1994 · $172,390

## Abstract

The goal for this period of support is to elucidate the actions and
interactions of luteinizing hormone-releasing hormone (LHRH), neuropeptide
Y (NPY), and gonadal steroids at the anterior pituitary (AP) to control
gonadotrophs (GTS) and luteinizing hormone (LH) and follicle-stimulating
hormone (FSH) secretion.  Studies will be conducted in vivo using a model
in which a neonatal or adult AP is allografted beneath the renal capsule of
an adult hypophysectomized (HYPX)-gonadectomized (GDX) host hamster.
Unanesthetized hosts will be administered LHRH and/or NPY by pulsatile
infusion through a venous cannula.  Some hosts will receive sc implants of
Silastic elastomer capsules containing testosterone (T), 5alpha-
dihydrotestosterone (DHT), or contain immunoreactive LH, FSH, or both
hormones using immunocytochemistry (ICC); size of LH and FSH cells using
morphometric techniques; mitotic activity of LH and FSH cells by injecting
hosts with 5-bromo-2'deoxyuridine and using ICC; T and E receptor
expression in GTS using ICC; ultrastructural features of LH cells using
electron microscopy and ICC; concentration and affinity of graft LHRH
receptors using ligand binding assays; LHalpha, LHbeta, and FSHbeta mRNA
expression in LH and FSH cells using in situ hybridization and ICC in flip-
flopped serial sections; relative levels of the subunit mRNAs in grafts
using blot hybridization, autoradiography, laser densitometric analysis,
and scintillation counting; and concentrations of LH, FSH, and their
subunits in grafts and plasma using radioimmunoassays.  These experiments
are designed to show how LHRH, NPY, and gonadal steroids interact directly
at the AP in vivo at a site distant from the immediate presence of the
hypothalamus and independent of the secretion of endogenous gonadal
factors.  The results are expected to clarify our understanding of the
roles these hormones play in postnatal development of GTS and in the
control of LH and FSH secretion in adult male and female hamsters.

## Key facts

- **NIH application ID:** 2198637
- **Project number:** 5R01HD022687-08
- **Recipient organization:** UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
- **SAM.gov UEI:** J22LNTMEDP73
- **Principal Investigator:** CHARLES A BLAKE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $172,390
- **Award type:** 5
- **Project period:** 1986-07-01 → 1996-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198637

## Citation

> US National Institutes of Health, RePORTER application 2198637, MECHANISMS OF CYCLIC LH AND FSH RELEASE (5R01HD022687-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198637. Licensed CC0.

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