# EXPRESSION OF IMMUNOGLOBULIN GENES IN TRANSGENIC MICE

> **NIH NIH R01** · UNIVERSITY OF CHICAGO · 1994 · $198,943

## Abstract

DESCRIPTION (Adapted from applicant's abstract):  Experiments are planned
for a detailed analysis of the regulation of the rearrangement of
immunoglobulin genes.  Previous work with transgenic mice has shown that
after rearrangement of a functional heavy and light chain gene a feedback
mechanism shuts off the further production of the Ig gene specific
recombinase.  1) It will now be determined whether the same mechanism
operates in all types of B cells, or whether two different B cell lineages
exist with respect to control mechanisms at the pre-B cell stage.  2) The
role of gamma 2b in feedback inhibition has been controversial, apparently
because the timing and/or quantities and/or membrane versus secreted forms
of transgenic gamma 2b varied in different transgenic lineages.  The
different transgenic gamma 2b lines will now be exploited to shed more
light on these parameters of the feedback control.  3) The isotypic
exclusion of kappa and lambda genes will be further studied by determining
the location of the lambda gene enhancer(s) and then producing
transgenic mice with a gene under control of its own enhancer.  4) In order
to define the molecular composition of the signal for feedback inhibition,
recombinase positive pre-cell lines will be transfected with modified
immunoglobulin genes and their effect on the recombinase will be
determined.  5) Individual pre-cells from Ig gene transgenic mice will be
analyzed for the timing of the expression of transgenic and endogenous Ig
genes using the polymerase chain reaction.  This may help unravel the
kinetics of the feedback response.  6) By targeting the lambda 5 or C mu
gene in embryonic stem cells with mutated gene constructs, mice will be
produced which lack lambda 5 or mu production in order to study the role of
these genes in early B cell development.

It is hoped that these experiments may help in defining the control of
immunoglobulin gene rearrangement and thus solve a well-known example of a
developmental control, as well as be of clinical importance for
immunological disorders with a B cell component.

## Key facts

- **NIH application ID:** 2198758
- **Project number:** 5R01HD023089-09
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **SAM.gov UEI:** ZUE9HKT2CLC9
- **Principal Investigator:** URSULA B STORB
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $198,943
- **Award type:** 5
- **Project period:** 1990-04-01 → 1996-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198758

## Citation

> US National Institutes of Health, RePORTER application 2198758, EXPRESSION OF IMMUNOGLOBULIN GENES IN TRANSGENIC MICE (5R01HD023089-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198758. Licensed CC0.

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