# GENE REGULATION IN SPERMATOGENESIS

> **NIH NIH R01** · TEXAS BIOMEDICAL RESEARCH INSTITUTE · 1994 · $138,928

## Abstract

The differentiation of specific cell types in mammals is mediated by
differential gene expression.  To understand the regulation of the
development and differentiation of a particular cell type, it is important
to understand what differential gene expression occurs in that cell type
and how this gene expression is regulated.  This proposal seeks to
understand the differentiation of spermatogenic cells by analyzing the
differential expression of one example gene, phosphoglycerate kinase-2
(Pgk-2).  The Pgk-2 gene is expressed only in spermatogenic cells and this
specific expression is regulated primarily at the transcriptional level.
The aims of this proposal are to define the components of the active Pgk-2
transcript ion complex as they occur in adult spermatogenic cells that
express the Pgk-2 gene.  These components will include protein
transcription factors that bind to specific sequences in the regulatory
region of the Pgk-2 gene, and specific chromatin states that must be
achieved in the Pgk-2 gene in order for the transcriptional process to
occur.  The final experiments proposed in this application are designed to
characterize the development of the active Pgk-2 transcription complex by
examining earlier, non-expressing cell types in the spermatogenic lineage
to determine when each component of the active complex becomes
established.  It is hoped that understanding of the components of the
active Pgk-2 transcription complex and how they are put together will
contribute to an understanding of how genes are regulated in spermatogenic
cells and ultimately how cases of defective gene expression in
spermatogenic cells might be treated.

## Key facts

- **NIH application ID:** 2198773
- **Project number:** 5R01HD023126-05
- **Recipient organization:** TEXAS BIOMEDICAL RESEARCH INSTITUTE
- **SAM.gov UEI:** J4EYPCJDQ1H6
- **Principal Investigator:** John R MCCARREY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $138,928
- **Award type:** 5
- **Project period:** 1987-04-01 → 1995-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198773

## Citation

> US National Institutes of Health, RePORTER application 2198773, GENE REGULATION IN SPERMATOGENESIS (5R01HD023126-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198773. Licensed CC0.

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