# THE ROLE OF BIOACTIVE FSH IN REPRODUCTION

> **NIH NIH R01** · STANFORD UNIVERSITY · 1994 · $147,277

## Abstract

Recent cloning of cDNAs for LH, FSH and TSH receptors indicated these
proteins belong to a sub-family of G-protein-coupled receptors with seven
transmembrane regions and a large extracellular domain.  We have recently
isolated cDNAs for both human LH and FSH receptors and expressed the
functional proteins capable of signal transduction in transfected cells.
Binding studies indicated that the human receptors, unlike their rodent
counterparts, have unique species-specific ligand recognition properties,
underlying the importance of using the human receptors to study human
physiology and pathophysiology.  Because truncated rat LH receptor with
only the extracellular region retains full ligand binding, we propose to
study the ligand binding and signal transduction of human LH and FSH
receptors with altered N-linked carbohydrates and peptide backbone in the
extracellular region.  Using a recently adapted RNA virus expression
system, we will express large amounts of the extracellular region of
recombinant human LH and FSH receptors with full ligand binding ability
for testing as receptor antagonists in vitro and in vivo.  Using the
human LH receptor and a reporter gene driven by a gonadotropinresponsive
promoter, we have developed a luminescence bioassay for human LH and CG.
We will use this bioassay to screen for defective hCG molecules during
spontaneous abortion.  Because auto-antibodies to TSH receptors have been
shown to be involved in different thyroid diseases, we propose to use
recombinant human gonadotropin receptors to screen for inhibitory and
stimulatory receptor autoantibodies in sera of patients with premature
ovarian failure and testotoxicosis, respectively.  The present proposal
should yield truncated human receptors capable of selectively
antagonizing the actions of human LH and FSH in patients,, as well as
further our understanding of diseases with defects of gonadotropins and
their receptors.

## Key facts

- **NIH application ID:** 2198809
- **Project number:** 5R01HD023273-08
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** AARON JW HSUEH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $147,277
- **Award type:** 5
- **Project period:** 1991-04-01 → 1997-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198809

## Citation

> US National Institutes of Health, RePORTER application 2198809, THE ROLE OF BIOACTIVE FSH IN REPRODUCTION (5R01HD023273-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198809. Licensed CC0.

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