# MALE GERM CELL DEVELOPMENT IN TRANSGENIC MICE

> **NIH NIH R37** · UNIVERSITY OF PENNSYLVANIA · 1994 · $241,029

## Abstract

The formation of mature germ cells in the adult male animal is a
unique developmental system.  Spermatogenesis requires the
maintenance of a stem cell pool which can self renew and can
undergo a complicated differentiation process to form the haploid
male gamete.  The introduction of new genes into mice provides a
powerful method by which to study this complicated process and
may reveal novel mechanisms for gene regulation.  We propose
studies in four areas.

1.  To identify the cis-acting regulatory elements required for
spermatid specific transcription of the mouse protamine-1 gene,
which codes for a unique protein involved in sperm chromatin
condensation.

2.  To determine the role of RNA sequences in the translational
regulation and stability of protamine message.

3.  To establish the degree of macromolecular movement between
syncytial spermatids during differentiation.

4.  To analyze two transgenic mouse lines which process distinct
characteristic defects in male germ cell development.

These experiments represent a new approach to the study of
spermatogenesis and will provide insight into the process of
differentiation and gene regulation.

## Key facts

- **NIH application ID:** 2198902
- **Project number:** 5R37HD023657-07
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **SAM.gov UEI:** GM1XX56LEP58
- **Principal Investigator:** Ralph Lawrence Brinster
- **Activity code:** R37 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $241,029
- **Award type:** 5
- **Project period:** 1988-02-01 → 1998-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198902

## Citation

> US National Institutes of Health, RePORTER application 2198902, MALE GERM CELL DEVELOPMENT IN TRANSGENIC MICE (5R37HD023657-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198902. Licensed CC0.

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