# CELL-MATRIX INTERACTIONS IN LIMB DEVELOPMENT

> **NIH NIH P01** · TUFTS UNIVERSITY BOSTON · 1993 · $713,709

## Abstract

The central theme of this proposal is the cellular and molecular events
that lead to differentiation of most of the major tissues of the
developing limb, namely cartilage, bone, tendon, muscle, and the
vasculature.  Project I will examine the role of a putative complex of
hyaluronan, hyaluronan-binding protein and an integrin in regulating
endothelial cell behavior, and the molecular nature of the protein
components of this complex.  Project II will continue to elucidate the
specific regulatory components involved in cartilage hypertrophy,
especially type X collagen, proteoglycans and other genes specifically
expressed during hypertrophy.  Project III will study the regulation of
a critical step in maturation of collagen fibers during tendon
development, also focussing on proteoglycan-collagen interactions and
expression of genes at the time of tendon maturation.  Project IV will
further study translation-inhibitory RNAs in the regulation of muscle
growth, and will also apply this concept to early differentiation events
in the limb.  The individual projects will employ a large variety of
technical approaches to achieve their aims; a major integrating factor
in the program is the sharing of the broad range of skills that have been
mastered by the members of the program in order to facilitate progress
in each project and to stimulate new experimental approaches.  These
studies will lead to a better understanding of morphogenesis and
differentiation during limb development, and will provide a more informed
basis for investigations of genetic and teratologically produced limb
anomalies.

## Key facts

- **NIH application ID:** 2198909
- **Project number:** 2P01HD023681-06
- **Recipient organization:** TUFTS UNIVERSITY BOSTON
- **SAM.gov UEI:** C1F5LNUF7W86
- **Principal Investigator:** BRYAN P TOOLE
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $713,709
- **Award type:** 2
- **Project period:** 1988-03-01 → 1998-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2198909

## Citation

> US National Institutes of Health, RePORTER application 2198909, CELL-MATRIX INTERACTIONS IN LIMB DEVELOPMENT (2P01HD023681-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2198909. Licensed CC0.

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