# ANALYSIS OF GROWTH CONTROL IN DEVELOPING MOUSE LIMBS

> **NIH NIH R29** · TULANE UNIVERSITY OF LOUISIANA · 1992 · $101,090

## Abstract

The long term objective for this project is to understand the
cellular processes involved in growth control and pattern
formation in developing mammalian limbs.  Experiments of the
type proposed have recently become possible due to the
development of techniques for in situ surgery on early stage
mouse embryos.  Experiments are proposed to begin a
developmental analysis of wildtype limbs and, in addition, limbs of
selected mutants in an effort to characterize alterations in the
normal course of growth which correlates with defective patterns.
Growth will be analyzed by utilizing a number of different
techniques including, BrdUrd incorporation to establish normal
growth parameters and cell type-specific birthdays; the
introduction of a marker gene to trace the fate of small
populations of cells in the limb bud; the use of growth specific
antibodies to define the pattern of growth termination during limb
development.  Finally, mutant limbs will be screened for
autonomy of the mutational effect by microsurgical grafting onto
wildtype host embryos.  The information gained from these
studies will ultimately be of significance to our understanding of
congenital birth defects, and  possibly to their correction.  In
addition, understanding how growth is controlled in the limb
during development may provide insight into how regenerative
growth may be enhanced in more mature mammalian limbs.

## Key facts

- **NIH application ID:** 2199008
- **Project number:** 5R29HD023921-05
- **Recipient organization:** TULANE UNIVERSITY OF LOUISIANA
- **SAM.gov UEI:** XNY5ULPU8EN6
- **Principal Investigator:** KEN MUNEOKA
- **Activity code:** R29 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $101,090
- **Award type:** 5
- **Project period:** 1988-03-01 → 1995-08-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199008

## Citation

> US National Institutes of Health, RePORTER application 2199008, ANALYSIS OF GROWTH CONTROL IN DEVELOPING MOUSE LIMBS (5R29HD023921-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199008. Licensed CC0.

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