# DIRECTED MUTAGENESIS OF THE HOX TWO AND AFP GENES

> **NIH NIH R01** · BAYLOR COLLEGE OF MEDICINE · 1994 · $249,870

## Abstract

In order to define the function of genes believed to be important in murine
development, the technique of homologous recombination will be applied to
mutate the Hox 2.1, Hox 2.6 and AFP genes in cultured ES cells.  It should
be possible to select or detect clones of cells which carry mutations
caused by homologous recombination within these genes using either
promoterless neo vectors and G418 selection, or MCLneo vectors and a tk or
hprt based negative selection scheme.

Selected clones carrying the appropriate genetic modification will be used
to construct chimaeras so that these specific genetic changes can be
introduced into the mouse germ line.  This will facilitate a direct
experimental test of the gene's function in development for the first time,
and will eventually lead to the genetic complementation analysis of these
loci.  The success of homologous recombination applied to the stem-cell
chimaera system will provide a general technique with which to develop
animal models of human genetic disease.

## Key facts

- **NIH application ID:** 2199247
- **Project number:** 5R01HD024613-05
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** ALLAN BRADLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $249,870
- **Award type:** 5
- **Project period:** 1989-12-01 → 1994-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199247

## Citation

> US National Institutes of Health, RePORTER application 2199247, DIRECTED MUTAGENESIS OF THE HOX TWO AND AFP GENES (5R01HD024613-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199247. Licensed CC0.

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