# ANALYSIS OF MOUSE DEVELOPMENT USING CELL LINES

> **NIH NIH R01** · HARVARD UNIVERSITY · 1994 · $339,951

## Abstract

DESCRIPTION:  This is a renewal application to continue work using gene
targeting and other modern molecular and genetic methods to study the
developmental regulation of early mammalian development. Emphasis will
be  placed on the 413.d insertional mutation and on the role of several
bone  morphogenetic proteins (BMP), BMP-5, BMP-6 and BMP-7.  413.d
appears to  control mesoderm formation in part because expression is
restricted to cells  surrounding the anterior tip of the primitive streak
(i.e., Hensen's node,  blastopore lip) and because mutant mice fail to
form mesoderm.  BMP proteins  are an important sub-family in the TGF-beta
family of genes.  This large  family has overlapping expression patterns
and presumably overlapping functions.  They are thought to be involved
in inductive processes and some are involved in dorso-ventral patterning.
By using a combination of knock-out  mutations, molecular markers for
cells and proteins, complementation with  spontaneous mutations, reporter
gene constructs, and other strategies, the  applicant proposes
comprehensive studies of the function of genes that  regulate early
stages of embryonic development.

## Key facts

- **NIH application ID:** 2199436
- **Project number:** 2R01HD025208-07
- **Recipient organization:** HARVARD UNIVERSITY
- **SAM.gov UEI:** LN53LCFJFL45
- **Principal Investigator:** ELIZABETH J ROBERTSON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $339,951
- **Award type:** 2
- **Project period:** 1989-01-01 → 1998-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199436

## Citation

> US National Institutes of Health, RePORTER application 2199436, ANALYSIS OF MOUSE DEVELOPMENT USING CELL LINES (2R01HD025208-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199436. Licensed CC0.

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