# INSERTIONAL MUTAGENESIS AND MOUSE DEVELOPMENT

> **NIH NIH R01** · BAYLOR COLLEGE OF MEDICINE · 1993 · $259,222

## Abstract

Early development will be studied in the mouse by the generation
of developmental mutants.  We will attempt to derive mutations both
in unknown genes, using random retroviral infection or embryonic
stem (ES) cells, as well as in two specific genes, c-myc and c-src,
using ES cells in which these genes have been disrupted by
retroviruses or homologous recombination.

To facilitate the analysis of insertional mutations in transgenic
mice, foreign DNA will be introduced into the germ line using
retroviral infection.  This method has been shown to produce
insertional mutants at a similar frequency to that observed using
microinjection of DNA into the zygote, but it offers the advantage
over the latter method of producing insertions with no
rearrangements of flanking sequences, which would otherwise
complicate further analysis.  The cloning of proviruses in mutant,
strains will be facilitated by retroviral vectors containing a
bacterial supF gene to allow selective cloning.  A rapid screen of
transgenic animals will be attempted by using retroviruses causing
microphthalmia of the eye, or by PCR analysis of blood samples.
The effect of parental imprinting on the transgene will also be
investigated.

To derive mutations in two specific genes, c-myc and c-src, ES
cells will he infected repeatedly with retroviruses and pools of
cells will be made.  Cells in which the provirus has inserted
within the gene will be identified using the polymerase chain
reaction (PCR).  These cells will be subcloned further prior to the
formation of germ line chimeras.  Alternatively, ES cells will be
selected for homologous recombination into the genes using the pMCl
or a promoterless neo selection system.

## Key facts

- **NIH application ID:** 2199499
- **Project number:** 5R01HD025326-05
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** ALLAN BRADLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $259,222
- **Award type:** 5
- **Project period:** 1989-07-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199499

## Citation

> US National Institutes of Health, RePORTER application 2199499, INSERTIONAL MUTAGENESIS AND MOUSE DEVELOPMENT (5R01HD025326-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199499. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*