# TRANSGENIC MICE WITH DEVELOPMENTAL DISORDERS

> **NIH NIH R01** · BAYLOR COLLEGE OF MEDICINE · 1993 · $138,676

## Abstract

The molecular mechanisms mediating morphogenesis during mammalian
embryogenesis are poorly understood.  In part this is related to
the fact that few mammalian genes known to control specific aspects
of morphogenetic development have been cloned and characterized.
The recent development of techniques to insert new genetic
information into the mouse genome and to create transgenic mice
provides a new methodology to simultaneously mutate and molecularly
tag mammalian genes.

The major goal of the research proposed herein is to develop a
vector system that significantly simplifies the search for, and
maintenance of, transgenic mice with developmental disorders caused
by insertional mutagenesis.  The specific aims are 1) to develop
a visual identification system (VlS) vector that will permit
hemizygous and homozygous transgenic mice to be identified and
distinguished by simple visual inspection, and then to sue the
vector to generate new families of transgenic mice which will be
screened for insertional developmental mutations; 2) to use the
VIS vector to evaluate the frequency of integration by homologous
recombination in microinjected mouse embryos; and 3) to use the VIS
vector to evaluate the frequency of integration by gap repair in
microinjected mouse embryos.

The VIS vector to be tested contains a lens-specific crystallin
promotor linked to a truncated, non-tumorigenic, SV40 early region.
The vector will be tested for dominant induction of cataract
formation, an easily identifiable visual phenotype.  The downless
gene will be the target for the homologous recombination
experiments and the OTCase gene will be used for the gap repair
experiments.

The identification by insertional mutagenesis of mammalians that
play specific roles in development, and the improvement of
techniques for gene-specific mutagenesis in mammalian organisms are
important steps toward a better understanding of the molecular
basis for mammalian embryogenesis and, morphogenesis.  Such studies
can have important implications for treatment of human infertility
and prevention of human birth defects.

## Key facts

- **NIH application ID:** 2199512
- **Project number:** 5R01HD025340-05
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **SAM.gov UEI:** FXKMA43NTV21
- **Principal Investigator:** Paul A. Overbeek
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $138,676
- **Award type:** 5
- **Project period:** 1989-04-01 → 1994-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199512

## Citation

> US National Institutes of Health, RePORTER application 2199512, TRANSGENIC MICE WITH DEVELOPMENTAL DISORDERS (5R01HD025340-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199512. Licensed CC0.

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