# MOUSE DEVELOPMENTAL MUTANTS DERIVED BY GENE TARGETING

> **NIH NIH R01** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 1993 · $237,291

## Abstract

We propose to generate, by gene targeting, mutant mice defective in cell
lineage determination and pattern formation.  Since early development in
mouse closely parallels human early development, these mutants will provide
models for studying causes of human birth defects as well as human
infertility problems resulting from early embryonic wastage and spontaneous
abortion.

Gene targeting, the homologous recombination of DNA sequences residing in
the chromosome with newly introduced DNA sequences, provides the means for
specifically altering the mouse genome. By using mouse embryo-derived stem
cells as the recipient cell line for the gene targeting experiments, the
means are available for transferring the mutations generated in cell
culture to living mice. The advantage of this scenario for creating
specific developmental defective mice compared to random mutagenic methods
is that on the one hand, the nature of the mutations at the discretion of
the experimentor and on the other hand, unlike random mutagenic methods,
the projected frequency of generating mice with specific mutations by gene
targeting is sufficiently high to make the procedure practical.

The particular mutant mice that we propose to generate will contain null
mutations in the int-1, int-2 and the homeo box genes hox 1.2 and hox 1.3.
This set of target genes was selected for our initial experiments because
they should yield a spectrum of defects in early development where a
correlation with altered cell lineage patterns should be evident. A genetic
analysis of these mutants should provide a deeper understanding of the
roles of these genes in controlling pattern formation. In the course of
these pioneering experiments, a major effort will also be devoted towards
developing practical strategies for generating mice of any desired mutant
genotype.

## Key facts

- **NIH application ID:** 2199530
- **Project number:** 5R01HD025395-05
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **SAM.gov UEI:** LL8GLEVH6MG3
- **Principal Investigator:** MARIO R CAPECCHI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $237,291
- **Award type:** 5
- **Project period:** 1989-05-01 → 1995-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199530

## Citation

> US National Institutes of Health, RePORTER application 2199530, MOUSE DEVELOPMENTAL MUTANTS DERIVED BY GENE TARGETING (5R01HD025395-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199530. Licensed CC0.

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