# REGULATORY ELEMENTS CONTROLLING TROPONIN I EXPRESSION

> **NIH NIH R01** · PURDUE UNIVERSITY · 1993 · $188,354

## Abstract

Skeletal muscle development involves the withdrawal of myogenic
stem cells from the cell cycle, the formation of multinucleated
muscle fibers and the coordinate accumulation of the contractile
proteins including alpha-actin, myosin heavy chain, myosin light
chain, alpha-tropomyosin, troponins C,I and T, and M-creatine
kinase.  Although these genes are expressed in a tissue-specific
fashion, the molecular regulatory mechanisms controlling their
developmental expression remain largely unknown.  As an initial
step in determining how the contractile protein gene set is
developmentally regulated, we have focused our efforts on
identifying the transcriptional regulatory mechanisms controlling
expression of the quail fast troponin I (TnI) gene (3).  Previous
studies demonstrated that the quail TnI gene, when introduced into
the mouse multipotential cell line C3H10T1/2 (85, 39, 55), exhibits
the correct developmental timing, tissue specificity and
quantitative levels of gene expression associated with the
endogenous mouse TnI gene (54, 53).  Subsequent experiments have
identified three regulatory regions that are responsible for this
skeletal muscle-specific expression pattern.  These include a 5'
quantitative regulatory element, an intronic tissue-specific
enhancer element and a 3' negative regulatory element.  To fully
characterize the TnI cis-acting regulatory regions and associated
trans-acting factors, we propose to utilize in vitro mutagenesis
strategies in conjunction with protein analysis and purification
schemes.  In addition, cDNA clones that express each of the TnI-
specific trans-acting regulatory factors will be isolated by lambda
gt11 library screening.  These experiments will provide novel
information on how TnI and other contractile protein genes are
developmentally regulated.  In addition, this study should produce
new insights into the molecular mechanisms that are responsible for
the differential transcription patterns observed in the developing
embryo.

## Key facts

- **NIH application ID:** 2199620
- **Project number:** 5R01HD025518-05
- **Recipient organization:** PURDUE UNIVERSITY
- **SAM.gov UEI:** YRXVL4JYCEF5
- **Principal Investigator:** STEPHEN F KONIECZNY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $188,354
- **Award type:** 5
- **Project period:** 1989-07-01 → 1995-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199620

## Citation

> US National Institutes of Health, RePORTER application 2199620, REGULATORY ELEMENTS CONTROLLING TROPONIN I EXPRESSION (5R01HD025518-05). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/2199620. Licensed CC0.

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