# OXYTOCIN-RECEPTOR-MEDIATED PROSTAGLANDIN RELEASE

> **NIH NIH R01** · UNIVERSITY OF TEXAS MED BR GALVESTON · 1993 · $123,511

## Abstract

Our long term goals are to understand the interaction between OT and PGs
and their importance in the initiation of labor.  The principal goal of
this proposal is to determine the relationship between the occupancy of OT
binding sites in decidua, endometrium and amnion and the production of PGs.
The specific aims are to:  1.  Study OT binding sites in decidua,
endometrium and amnion with respect to affinity, concentration, ligand
specificity, and metal ion requirements.  2.  Show the relationship between
OT binding and PG release in these tissues, with respect to OT agonists and
antagonists.  3.  Study the relationship between changes in OT sensitivity
of PG release and changes in the concentration of OT receptors.  We will
study decidua from guinea pigs and rabbits at different stages of pregnancy
and guinea pig endometrium during the estrous cycle.  4.  Study effects of
estrogen and progesterone, administered both in vivo and in vitro, on OT
receptor concentrations in guinea pig and rabbit endometrium and cultured
human amnion cells.  We hope to establish whether steroid-induced changes
in receptor concentration are related to changes in the sensitivity of the
release of PGs.  5.  Establish the validity of using an iodinated OT
antagonist, previously shown to bind specifically to OT receptors in.  the
myometrium, mammary gland and regions of the brain, as a probe for OT
receptors in the endometrium, decidua, and amnion.  The use of iodinated
antagonist would greatly reduce the amount-of tissue needed for subsequent
studies.  6.  Study physicochemical properties of OT receptors in the
endometrium/decidua and possibly amnion.  These studies include
determination of the functional molecular mass by radiation inactivation
studies, the size of the binding unit by photoaffinity cross-linking and
solubilization studies, as carried out by us previously with OT receptors
in the rat mammary gland.  These studies will form the basis for later work
elucidating the mechanisms by which OT stimulates PG production.

## Key facts

- **NIH application ID:** 2199848
- **Project number:** 5R01HD026168-06
- **Recipient organization:** UNIVERSITY OF TEXAS MED BR GALVESTON
- **SAM.gov UEI:** MSPWVMXXMN76
- **Principal Investigator:** MELVYN STANLEY SOLOFF
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $123,511
- **Award type:** 5
- **Project period:** 1989-08-01 → 1995-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199848

## Citation

> US National Institutes of Health, RePORTER application 2199848, OXYTOCIN-RECEPTOR-MEDIATED PROSTAGLANDIN RELEASE (5R01HD026168-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199848. Licensed CC0.

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