# DEVELOPMENTAL BIOLOGY OF PROTEIN TRYOSINE KINASES

> **NIH NIH R29** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 1994 · $131,503

## Abstract

Protein tyrosine kinases are thought to be involved in the processes of
cell division, migration and differentiation occurring during embryonic
development, but their physiological functions, regulatory properties and
mechanism of action are largely unknown.  Proteins phosphorylated on
tyrosine have been detected in embryonic tissues using antibodies specific
for phosphotyrosine residues.  However, a large fraction of the protein
tyrosine kinases involved in developmental processes may not be known at
this time.  Using anti-phosphotyrosine antibodies to screen chicken embryo
cDNA libraries will allow the isolation of the tyrosine kinase genes that
are expressed during development.  The cloned protein tyrosine kinases will
then be identified by sequencing the appropriate cDNAs and characterized
using specific polyclonal antibodies to detect them in embryonic tissues or
cultured cells.  Antibodies will be prepared by injecting the cloned
kinases expressed in bacteria as fusion proteins and used in
immunoprecipitation, immunoblotting and immunofluorescence microscopy
experiments.  Finally, the effects that one of the newly identified
tyrosine kinases has on cellular growth and differentiation will be
investigated by producing eukaryotic cell lines that express the chosen
tyrosine kinase cDNA, in its wild type and mutated forms, and by
characterizing their phenotypes.  The DNA and antibody probes generated in
the course of the proposed project will be useful for further studies
leading to a detailed characterization of protein tyrosine kinases and
their role in embryonic development.  The cloning and characterization of
novel protein tyrosine kinases may also contribute to the recognition of
new oncogenes encoding tyrosine kinases and of their roles in the events
leading to oncogenic transformation.

## Key facts

- **NIH application ID:** 2199906
- **Project number:** 5R29HD026351-06
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **SAM.gov UEI:** PHMKYKKJLQS1
- **Principal Investigator:** ELENA B PASQUALE
- **Activity code:** R29 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1994
- **Award amount:** $131,503
- **Award type:** 5
- **Project period:** 1990-03-01 → 1995-09-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199906

## Citation

> US National Institutes of Health, RePORTER application 2199906, DEVELOPMENTAL BIOLOGY OF PROTEIN TRYOSINE KINASES (5R29HD026351-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199906. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*