# NEURAL INDUCTION--ANALYSIS USING MONOCLONAL ANTIBODIES

> **NIH NIH R01** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 1993 · $105,891

## Abstract

Neural induction has been defined operationally, and the interacting
tissues have been grossly identified, but the molecular mechanisms of
neural induction are not known.  The ultimate aims of this proposal are to
define those mechanisms at cellular, molecular and genetic levels in the
frog, Xenopus.  The initial aim is to isolate components of the gastrula
that have neural inductive potency which can be used as immunizing material
for production of monoclonal antibodies.  A novel method of retrieval of
such material is used:  a nitrocellulose filter is implanted in the dorsal
blastopore lip region during neural induction.  After about 4 hours the
conditioned filter is removed and cut in half: piece (A) is tested for
inductive potency by ventral implantation into an early gastrula (controls
show that virgin nitrocellulose does not have an effect); piece B is stored
frozen.  If (A) proves to have neural inductive potency, (B) is used as
immunizing material in mice that have been tolerized from birth by
injections of ventral halves of Xenopus gastrulae (Hockfield 1987).  The
aim is to produce monoclonal antibodies that react specifically with
components of the neural induction system attached to nitrocellulose
filters.  To identify these components, antibodies are screened on tissue
sections of different stage Xenopus embryos, and by SDS PAGE and Western
blotting.  Antibodies are tested for their ability to block the neural
induction potency of conditioned filters, and to block neural induction in
vivo and in vitro, assayed by NCAM expression (Jacobson and Rutishauser
1986).  Suitable antibodies are used to screen a Xenopus gastrula cDNA
expression library, with the aim of identifying proteins involved in neural
induction.

## Key facts

- **NIH application ID:** 2199958
- **Project number:** 5R01HD026460-04
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **SAM.gov UEI:** LL8GLEVH6MG3
- **Principal Investigator:** MARCUS JACOBSON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $105,891
- **Award type:** 5
- **Project period:** 1990-01-01 → 1994-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199958

## Citation

> US National Institutes of Health, RePORTER application 2199958, NEURAL INDUCTION--ANALYSIS USING MONOCLONAL ANTIBODIES (5R01HD026460-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199958. Licensed CC0.

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