# PROTO-ONCOGENES IN GAMETOGENESIS AND EARLY DEVELOPMENT

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1993 · $305,724

## Abstract

The overall goal of the proposed research is investigation of the
potential roles of proto-oncogenes in mammalian gametogenesis and early
embryonic development. The c-mos c-abi and int-1 proto-oncogenes are
transcribed during specific stages of spermatogenesis in the mouse. In
addition, c-mos is transcribed in growing oocytes and high levels of c-
mos RNA are accumulated by the germinal vesicle stage. The oocyte c-mos
RNA is post-transcriptionally polyadenylated in concert with oocyte
maturation and is degraded by the mid to late two cell stage, suggesting
possible roles for c-mos as a maternal message. Since c-mos represents
the first candidate for a regulatory maternal RNA unique to oocytes,
further studies with its expression in spermatogenesis. Synthesis,
phosphorylation and stability of the c-mos protein will be studied in
spermatocytes, oocytes, eggs and embryos. The biological functions of c-
mos in oocytes will be investigated by inhibiting c-mos expression using
anti-sense oligonucleotides and/or anti-mos antibodies. These studies
will be correlated with alterations in protein phosphorylation to
identify candidate targets for c-mos protein kinase activity. Regulatory
sequences and trans-acting factors which control c-mos expression during
maturation of spermatocytes and oocytes will be investigated and the
developmental specificity of c-mos expression in spermatogenesis will be
further studied. The expression of additional selected proto-oncogenes
will be studied in spermatogenesis, oogenesis and pre-implantation mouse
embryos. These studies of normal proto-oncogene function in development
will also contribute to understanding the mechanisms by which oncogenes
induce neoplastic transformation.

## Key facts

- **NIH application ID:** 2199993
- **Project number:** 5R01HD026594-05
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** GEOFFREY M COOPER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $305,724
- **Award type:** 5
- **Project period:** 1989-08-01 → 1995-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/2199993

## Citation

> US National Institutes of Health, RePORTER application 2199993, PROTO-ONCOGENES IN GAMETOGENESIS AND EARLY DEVELOPMENT (5R01HD026594-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/2199993. Licensed CC0.

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