# DEVELOPMENTAL CONTROL OF MYOSIN LIGHT CHAIN GENES

> **NIH NIH R01** · BOSTON UNIVERSITY MEDICAL CAMPUS · 1989 · $187,777

## Abstract

In rat fast skeletal muscle, two light chain myosin proteins, LC1 and LC3,
are expressed from a single genetic unit.  The unique structures of the LC1
and LC3 proteins are generated by differential transcription from two
promoters separated by 10kb, accompanied by two alternate splicing
pathways.  In fetal muscle development, and in differentiating myogenic
tissue culture cells, accumulation of the LC1 mRNA precedes that of LC3,
suggesting that differential transcription and splicing of LC1 and LC3
messages is developmentally regulated.

Our objective is to characterize the mechanisms by which differentiating
muscle tissue generates two different myosin light chain transcripts from
the same genetic locus.  By testing isolated LC1 and LC3 promoters in
available tissue culture systems for the ability to express heterologous
test genes upon cellular differentiation, we will determine the extent to
which upstream regulatory elements play a role in the selective
transcription of LC1 and LC3 mRNAs during the differentiation process.

## Key facts

- **NIH application ID:** 3120704
- **Project number:** 9R01AG008920-04
- **Recipient organization:** BOSTON UNIVERSITY MEDICAL CAMPUS
- **SAM.gov UEI:** FBYMGMHW4X95
- **Principal Investigator:** Nadia A Rosenthal
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $187,777
- **Award type:** 9
- **Project period:** 1985-08-01 → 1992-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3120704

## Citation

> US National Institutes of Health, RePORTER application 3120704, DEVELOPMENTAL CONTROL OF MYOSIN LIGHT CHAIN GENES (9R01AG008920-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3120704. Licensed CC0.

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