# PROTEOGLYCAN SYNTHESIS DURING DEVELOPMENT AND AGING

> **NIH NIH R01** · CASE WESTERN RESERVE UNIVERSITY · 1991 · $190,142

## Abstract

We propose the continuation of experimentation directed at understanding
the various control elements involved in the change of biosynthesis of
cartilage proteoglycans during various aspects of development and aging of
chondrocytes.  Our primary focus will be on the characterization of
proteoglycans produced by cells undergoing the transition between
undifferentiated limb mesenchymal cells into newly committed chondrocytes.
In addition, we propose to study the detailed biosynthesis of proteoglycans
as chondrocytes progress through the expressional stages of their
development history into senescence.  Emphasis will be on the
characterization and detailed chemistry of the core protein of cartilage
proteoglycan with respect to how it interacts with the total biosynthetic
pathway for proteoglycans.  Such considerations must focus on the elements
involved in the construction, maintenance and turnover of the extracellular
matrix associated with chondrocytes.  The studies proposed here deal with
chondrocytes derived from chick embryonic and adult cartilages.
Experimental characterization of the core protein of proteoglycans isolated
from various sources involves a combination of immunological probes and
detailed peptide fingerprinting.  With the availability of this technology,
we have outlined 6 individual projects.  The experimentation represents a
continuation of the technical procedures now current in this laboratory as
well as the emphasis of new, recently acquired techniques involving use of
antibodies and limited proteolysis to obtain analytical and qualitative
information regarding the protein moieties associated with proteoglycan.
The central theme of our research efforts is to provide insight into the
molecular events involved in the commitment of mesenchymal cells into
chondrocytes and the details involved in the expressional progression from
an embryonic to a senescent cell.

## Key facts

- **NIH application ID:** 3120717
- **Project number:** 5R01AG008932-11
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **SAM.gov UEI:** HJMKEF7EJW69
- **Principal Investigator:** Arnold Irwin Caplan
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $190,142
- **Award type:** 5
- **Project period:** 1989-02-01 → 1994-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3120717

## Citation

> US National Institutes of Health, RePORTER application 3120717, PROTEOGLYCAN SYNTHESIS DURING DEVELOPMENT AND AGING (5R01AG008932-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3120717. Licensed CC0.

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