# MAJOR HISTOCOMPATIBILTY COMPLEX--AGING & TRANSGENIC MICE

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1990 · $209,387

## Abstract

We have generated much evidence of both a "direct" and an "indirect"
nature that the major histocompatibility complex (MHC) influences the
aging rate, and some evidence that these effects map to the D and possibly
K-A regions of H-2 in mice.  Aim now is to carry forward studies of the
relation of the MHC to aging by making transgenic Mus with genes cloned
from the MHC of the long-lived (7-8 year) rodent Peromyscus leucopus.
Genes from the D-end-like segment of Peromyscus MHC will utilized,
including class I genes, TNF, and gene sequences from the same region
whose possible functions are not yet specified but may relate to "non-
immune" aspects of the MHC.  The peromyscus-> Mus transgenics will be
studied for Peromyscus MHC gene and gene product expression and function
by neurological technics (immunoprecipitation, flow cytometry, restriction
in cytotoxicity), for reproductive senescence, life span and diseases
(especially tumor incidences), mRNA levels in several organs for SOD,
catalase, mixed function oxidases, class I and II antigens, and tumor
necrosis factor (all of which are known to be related to both aging and to
the MHC), and repair of DNA adducts following injection of benzo
(a)pyrene-trans-7,8-diol, which pilot studies suggests are also both age
and MHC related.  Further inbreeding of Peromyscus and serologic and
molecular genetic studies of Peromyscus MHC will also be done, as adjunct
to the above studies.

## Key facts

- **NIH application ID:** 3120722
- **Project number:** 1R01AG008936-01
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** ROY L. WALFORD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $209,387
- **Award type:** 1
- **Project period:** 1990-08-01 → 1993-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3120722

## Citation

> US National Institutes of Health, RePORTER application 3120722, MAJOR HISTOCOMPATIBILTY COMPLEX--AGING & TRANSGENIC MICE (1R01AG008936-01). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3120722. Licensed CC0.

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