# ACTIVATORS OF HUMAN MUSCLE GENES

> **NIH NIH R01** · STANFORD UNIVERSITY · 1990 · $202,055

## Abstract

The proposed studies are intended to increase our understanding of the
mechanisms underlying muscle differentiation.  We previously demonstrated
the existence of diffusible trans-acting activator(s) of muscle genes in
studies involving heterokaryons, stable somatic cell hybrids formed between
mouse muscle and human nonmuscle cells.  Our goal is to characterize the
macromolecule(s) responsible for muscle gene activation.  Toward this end
we will obtain nuclear and cytoplasmic subfractions from mouse muscle
cultures at different stages of differentiation and microinject these
cellular components into human nonmuscle cells.  The activation of a
specific human muscle gene coding for a cell surface antigen present
throughout myogenesis will be monitored at the single cell level.
Eventually requirements for the expression of five temporally distinct
markers of human muscle differentiation will be examined.  In this manner
we hope to characterize the species of cytoplasmic macromolecule(s)
responsible for muscle gene activation and whether the same macromolecules
regulate the expression of muscle functions specific to different stages of
differentiation.

## Key facts

- **NIH application ID:** 3121371
- **Project number:** 9R01AG009521-04
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** Helen M Blau
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $202,055
- **Award type:** 9
- **Project period:** 1986-04-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3121371

## Citation

> US National Institutes of Health, RePORTER application 3121371, ACTIVATORS OF HUMAN MUSCLE GENES (9R01AG009521-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3121371. Licensed CC0.

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