# AGE INFLUENCE ON ISCHEMIA REPERFUSION IN BRAIN

> **NIH NIH R01** · OKLAHOMA MEDICAL RESEARCH FOUNDATION · 1991 · $147,348

## Abstract

Stroke occurs more frequently as age increases.  We have shown that an
ischemia/reperfusion insult (IRI) to brain of Mongolian gerbils causes
lethality in old gerbils significantly more than in younger animals.  We
have shown using four separate approaches including two exogenous traps,
salicylate and phenyl-t-butyl nitrone (PBN), as well as measurement of
brain protein oxidation that oxidative damage does occur in the
IRI-lesioned brain.  In addition, the spin-trap, PBN, protected animals
from brain injury from an lRI.  Our preliminary data allow us to
postulate that crucial oxidation-mediated lesions occur in the old gerbil
brain which makes it less able to withstand the massive oxidative stress
and disproportionate demand on energy production requirement brought on
by an lRI.  To test this hypothesis, we will use in vivo trapping of free
radicals, using salicylate and the spin-trap PBN, as well as brain
protein oxidation to determine if an IRI produces crucial oxidative
damage in old gerbils as compared to younger animals. (31)P-NMR
spectroscopy will be used to monitor the metabolic history of each
treated gerbil.  We will determine if the IRI induced spin trapped free
radicals are different in old versus younger gerbils.  Oxidative damage
to gerbil brain DNA and RNA will be assessed by measuring the
8-hydroxyguanine content and particular attention will be directed toward
synaptosomal DNA and RNA.  Older gerbil brains peroxidize less than
younger ones yet peroxidation impairs endogenous respiration in old brain
synaptosomes but not those from younger animals.  We will assess
propensity of IRI-lesioned gerbil brain to peroxidize during the
reperfusion phase to determine if brain from older animals show
significantly different time-course patterns as compared to younger
animals.  All of the proposed studies should help explain why old animals
are more susceptible to an IRI.

## Key facts

- **NIH application ID:** 3121608
- **Project number:** 1R01AG009690-01
- **Recipient organization:** OKLAHOMA MEDICAL RESEARCH FOUNDATION
- **SAM.gov UEI:** NGCNCJ1X6XA4
- **Principal Investigator:** ROBERT A FLOYD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $147,348
- **Award type:** 1
- **Project period:** 1991-05-01 → 1996-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3121608

## Citation

> US National Institutes of Health, RePORTER application 3121608, AGE INFLUENCE ON ISCHEMIA REPERFUSION IN BRAIN (1R01AG009690-01). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3121608. Licensed CC0.

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