# MAMMALIAN TUBULIN ISOTYPES & THEIR INTERACTION WITH MAPS

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1993 · $249,448

## Abstract

This proposal concerns the structure, evolution and expression of mammalian
tubulin genes.  These genes encode the Alpha- and Beta-subunits of
microtubules, structures that perform an essential role in many vital
organelles--cilia, flagella, the mitotic spindle and the cytoskeleton--of
all eucaryotic cells.  The proposed approach will involve 1)
characterization by DNA sequence analysis of human Beta-tubulin genes that
encode distinct Beta-tubulin isotypes.  2)a) Synthesis of peptide haptens
corresponding to small carboxyterminal regions that are unique to
individual Beta-tubulin isotypes, b) use of these haptens in hapten/carrier
conjugates for the generation of Beta-tubulin isotype-specific monoclonal
antisera, c) application of these sera in immunofluorescence experiments to
determine whether two or more Beta-tubulin isotypes can coassemble in a
single microtubule.  3)a) Isolation of cDNA clones encoding mouse Alpha-
and Beta-tubulin sequences from cDNA libraries constructed from whole
embryo and adult polyA+ mRNA, b) generation of gene-specific
3'-untranslated region subcloned probes, c) use of these probes in in situ
hybridization experiments using cryostat sections of staged animals to
study the tissue-specific developmental regulation of individual tubulin
genes.  4) Use of Beta-tubulin gene/BPV shuttle vectors for the
identification of sequences within human Beta-tubulin genes that are
critical in determining changes in the level of cytoplasmic Beta-tubulin
mRNAs that occur in response to modulation of the size of intracellular
monomer pools.  5)a) Construction of cloned probes encoding Tau and MAP
sequences, b) use of these probes i) to determine the number and
evolutionary conservation of the sequences encoding these proteins, ii) to
assess the response of Tau and/or MAP mRNA levels to changes in the level
of tubulin monomer, iii) to study the developmental regulation of these
proteins by in situ hybridization.

## Key facts

- **NIH application ID:** 3121937
- **Project number:** 5R01AG009989-11
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** NICHOLAS COWAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $249,448
- **Award type:** 5
- **Project period:** 1982-09-01 → 1996-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3121937

## Citation

> US National Institutes of Health, RePORTER application 3121937, MAMMALIAN TUBULIN ISOTYPES & THEIR INTERACTION WITH MAPS (5R01AG009989-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3121937. Licensed CC0.

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