# FIBRONECTIN AND CELL RECRUITMENT

> **NIH NIH R01** · STATE UNIVERSITY NEW YORK STONY BROOK · 1992 · $166,342

## Abstract

Fibronectin, a 440,000 dalton glycoprotein produce by fibroblasts,
monocytes, and epithelial and endothelial cells, circulates in human plasma
at concentrations of 300 g/ml; associates with fibrin upon activation of
the clotting system; and is deposited at sites of inflammation and wound
healing.  The fibronectin deposition in these sites is both plasma-derived
and cell-derived and is associated with proliferating migrating and
localizing cell populations including monocyte/macrophage, endothelial
cells, epidermal cells and fibroblasts.  In vitro plasma or cellular
fibronectin can bind to a substratum of collagen or fibrinogen and
simultaneously bind to fibroblasts.  These data taken together suggeest
that fibronectin may provide a critical link between cells and their
substrata especially during tissue remodeling.  We propose to test this
hypothesis by investigating how plasma and cellular fibronectin or
fibronectin fragments affect normal human cell adherence and chemotaxis in
vitro and cell localization in vivo.  We have developed quantitative,
reproducible assays for study of human cell adherence to determine whether
normal human cells can utilize fibronectin for their attachment as do
cell-lines and to study whether the requirement for fibronectin is the same
among the various human cell types.  To address the latter issue multiple
parameters in the adherence assays will be varied to delineate time course
for adherence, tenacity of adherence, optimal amounts of fibronectin for
adherence and other optimal conditions for adherence of each cell type (Aim
1).  We will also study epidermal cell adherence on fibroblast and
epidermal cell derived substrates (Aim 1).  Since we have already
demonstrated that fibronectin enhances monocyte, and epidermal cell
adherence, we will investigate mechanisms by which this may occur by
altering the protein (Aim 2) and modifying the cell (Aim 3).  In addition,
we will investigate whether fibronectin or fibronectin fragments recruit
cells by means other than cell adherence.  Specifically, we have shown that
the 120K and 210K fibronectin fragments stimulate monocyte chemotaxis and
will study possible mechanism(s) by which chemoattractive fibronectin
fragments may be generated in vivo and will define the limiting peptide
sequence necessary for chemotactic activity (Aim 4).  In addition, we will
determine the effect of fibronectin and other ECM proteins on epidermal
cell motility (Aim 5).  Finally, we will investigate whether fibronectin or
fibronectin fragments recruit cells in vivo (Aim 6).  Our ultimate goal is
to understand the role of fibronectin in wound healing.

## Key facts

- **NIH application ID:** 3122103
- **Project number:** 5R01AG010143-10
- **Recipient organization:** STATE UNIVERSITY NEW YORK STONY BROOK
- **SAM.gov UEI:** M746VC6XMNH9
- **Principal Investigator:** RICHARD August CLARK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $166,342
- **Award type:** 5
- **Project period:** 1982-07-01 → 1996-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3122103

## Citation

> US National Institutes of Health, RePORTER application 3122103, FIBRONECTIN AND CELL RECRUITMENT (5R01AG010143-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3122103. Licensed CC0.

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