# RFLP-MAPPING OF QTLS FOR LIFE SPAN AND LIFE HISTORY

> **NIH NIH R01** · UNIVERSITY OF COLORADO · 1992 · $169,907

## Abstract

We propose to establish a complete linkage map in the nematode
Caenorhabditis elegans based on restriction fragment length
polymorphisms (RFLPs). We intend to focus especially on those RFLPs
generated by transposable elements (Tc1 and Tc2).  The linkage map will
then be used to map quantitative trait loci (QTLs) specifying life span
and other life history traits.  Subsequent integration of the RFLP map
with extant physical and genetic maps will allow simultaneous
positioning of QTLs onto genetic maps, making possible the directed
construction of stocks showing specific variations in life span and
other life history traits, and will simplify the eventual molecular
characterization of genes specifying these traits.

Although such positioning has been a theoretical possibility for several
years, four new developments make the project feasible and of
considerable importance: (1) the construction of overlapping arrays of
contiguous DNA sequences or "contigs" [Coulson et al. (1988) Nature,
335:184.]; (2) an effective mathematical algorithm for assigning QTLs to
various physical regions (Lander and Botstein (1989) Genetics, 121:185]
which has already been used to map QTLs in the tomato [Paterson et al.
(1988) Nature, 335:721); (3) the development of recombinant inbred (RI)
lines in the nematode C. elegans [Johnson (1987) Proc.  Natl.  Acad.
Sci.  USA, 84:3777]; and (4) a procedure for resolving genomic
restriction digests on 2-D gels that may allow the simultaneous scoring
of hundreds of RFLPs [Uitterlinden et al. (1989) Proc.  Natl.  Acad.
Sci. USA, 86:2742].

## Key facts

- **NIH application ID:** 3122197
- **Project number:** 5R01AG010248-02
- **Recipient organization:** UNIVERSITY OF COLORADO
- **SAM.gov UEI:** SPVKK1RC2MZ3
- **Principal Investigator:** Thomas E. Johnson
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $169,907
- **Award type:** 5
- **Project period:** 1991-06-01 → 1994-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3122197

## Citation

> US National Institutes of Health, RePORTER application 3122197, RFLP-MAPPING OF QTLS FOR LIFE SPAN AND LIFE HISTORY (5R01AG010248-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3122197. Licensed CC0.

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