# IDENTIFICATION OF LH-RECEPTOR GENE REGULATORY SEQUENCES

> **NIH NIH R01** · UNIVERSITY OF WYOMING · 1993 · $160,815

## Abstract

The long term goal of this proposed research is to elucidate the
regulation of the rat LH receptor gene.  The immediate objectives are to
define the promoter region and to identify regulatory elements in this
gene.  Evidence for the critical roles of LH, hCG and their receptor in
female reproductive physiology is compelling.  A failure in expression of
these hormones or the receptor will results in no ovulation and no
pregnancy, leading to infertility.  Transcription of the LH receptor gene
can be induced by FSH or cAMP/estrogen and the expressed receptor can be
suppressed by LH/hCG.  Genes that are not only inducible but also
suppressible are interesting as is the LH receptor gene. because they are
likely to be involved in the switching mechanisms of important biological
processes.
     Studies on these hormone-dependent inducibility and suppressibility
could reveal novel enhancer and suppressor elements and will serve as a
model for other protein hormone receptors.  Understanding transcriptional
control necessitates the identification of regulatory elements.  Our
preliminary study suggests that [1] a single gene encodes the rat LH
receptor, [2] 11 exons are present In the coding region, [3] there are a
sequence similar to the ERE. an exact and several homologous AP-2, and a
G-rich CRE. but no consensus CRE, and [4] there are variant receptors
that appear to be produced by alternate splicing.  These results serve as
the basis for proposed studies concerning the following major goals.

1. We will determine the size and restriction map of the entire gene, the
transcription start-sites and the boundaries of an apparent intron in the
5'UTR.
2. The cAMP- and estradiol-responsive enhancer elements and LH-responsive
suppressor elements in the rat LH-R gene will be identified and verified.
3. We would like to determine whether FSH- or cAMP/estradiol-induction of
LH receptor transcription is direct or requires de novo protein
synthesis.
4. We plan to characterize variant mRNAs which expect to produce a
soluble form of receptors without a transmembrane domain.  These soluble
receptors may play important roles in the regulation of the hormone
concentration in serum.

## Key facts

- **NIH application ID:** 3122457
- **Project number:** 5R01AG010559-03
- **Recipient organization:** UNIVERSITY OF WYOMING
- **SAM.gov UEI:** FDR5YF2K32X5
- **Principal Investigator:** Tae H. Ji
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $160,815
- **Award type:** 5
- **Project period:** 1991-08-01 → 1994-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3122457

## Citation

> US National Institutes of Health, RePORTER application 3122457, IDENTIFICATION OF LH-RECEPTOR GENE REGULATORY SEQUENCES (5R01AG010559-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3122457. Licensed CC0.

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