# A GENETICALLY TRACTABLE IN VITRO MODEL OF AD

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1991 · $116,682

## Abstract

It is imperative that an in vitro model of Alzheimer's Disease be
developed; this would greatly improve studies of the mechanism(s) of
Alzheimer's disease and allow screening of potential therapeutic agents.
In addition, if the model were genetically manipulable, then the gene or
genes important for the development of the Alzheimer's pathology could be
determined.

No such model has been available previously, but data presented in this
proposal suggest that an in vitro model of Alzheimer's disease has now been
developed (see Preliminary Results).  This consists of long-term
reaggregating brain cultures of trisomy 16 mouse central nervous system.
It is proposed that these studies be extended by (1) characterizing the
reaggregating brain cultures of Ts16 mice and control littermates for beta-
amyloid production, abnormally phosphorylated tau (i.e., the mouse
equivalent of the Alz-50 target), phosphorylated neurofilament, neuronal
density vs. time, paired helical filaments, and APP751/APP695 mRNA ratios;
and (2) producing temperature-sensitive immortalized T16 neural and glial
cells, then transfecting the cell with a reporter expression construct and
including the cells in the reaggregating cultures.

The long-term goal of this project is to develop and characterize an in
vitro model of Alzheimer's disease that will be genetically manipulable.
This should allow an evaluation of the region of mouse chromosome 16 that
is important for the development of the changes associated with Alzheimer's
disease, and, ultimately, determination of the important gene or genes.

## Key facts

- **NIH application ID:** 3122598
- **Project number:** 1R01AG010671-01
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** Dale E. Bredesen
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $116,682
- **Award type:** 1
- **Project period:** 1991-09-29 → 1994-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3122598

## Citation

> US National Institutes of Health, RePORTER application 3122598, A GENETICALLY TRACTABLE IN VITRO MODEL OF AD (1R01AG010671-01). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3122598. Licensed CC0.

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